Specificity & Preparation: The antibody was purified by protein-A affinity chromatography from mouse ascites fluid. It will recognize C-terminal, N-terminal, or internal 6-His epitopes, with very high sensitivity and low background. This antibody is routinely tested by western blot.

Usage: Applications include immunoblotting (western blot 1:1,000-5,000; dot blot), immunoprecipitation, immunostaining, and ELISA.

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Specificity & Preparation: Recognizes proteins acetylated on lysine residues. Tested: acetylated histone, acetylated BSA, and acetylated MBP, no reaction to the non-acetylated proteins. Purified with immobilized acetylated lysine on agarose. Conjugated to d-Biotin via amine acylation.

Usage: ELISA (1:1000-5000), Western Blot (1: 250-1000), and immunoprecipitation (10 µg/1 mg proteins).

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Specificity & Preparation: Recognizes proteins acetylated on lysine residues. Tested: acetylated histone, acetylated BSA, and acetylated MBP, no reaction to the non-acetylated proteins. Purified with immobilized acetylated lysine on agarose.

Usage: ELISA, Western Blot, and immunoprecipitation.

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Specificity & Preparation: This antibody recognizes mammalian ACTH. The immunogen was raised in rabbits against the synthetic human ACTH 1-39. The antibody is routinely tested by dot blot.

Usage: Applications include immunostaining (1:200 to 1:600), immunoblotting (1:500), immunohistochemistry (fresh or frozen non-fixed sections) and radioimmunoassay.

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Specificity & Preparation: This antibody targets activated human matrix metalloproteinase 9 (MMP-9) in homo sapiens, oryctolagus cuniculus, canis familiaris, Sus scrofa. The immunogen was ovalbumin-conjugated synthetic peptide; acetylated-FQTFEGDLK (N-terminal sequence common to human, rabbit, dog, pig) in BALB-c mouse.

Usage: Applications include immunoblotting (western undiluted) and immunohistochemistry (undiluted, no pretreatment necessary).

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Specificity & Preparation: Recognizes phosphorylated Akt on serine 473. Does not cross-react with the non-phosphorylated Akt. Affinity-purified with phospho-peptide affinity chromatography followed by affinity absorption with non-phosphopeptide.

Usage: ELISA (0.25 µg/mL), Western Blot (1 µg/mL), and immunoprecipitation (10 µg/2 mg crude protein).

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Specificity & Preparation: Recognizes phosphorylated Akt1 on serine 473. Does not cross-react with the non-phosphorylated form of Akt. Affinity-purified with phospho-peptide affinity chromatography followed by affinity absorption with non-phosphopeptide of the same sequence.

Usage: ELISA (0.25 µg/mL) and Western Blot (0.25-1 µg/mL).

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Specificity & Preparation: Recognizes PKB beta, does not cross-react with PKB alpha and gamma. Immunoaffinity purified with PKB beta peptide (ASIRE) on Agarose.

Usage: ELISA (0.25 µg/mL) and Western Blot (1:1000).

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Specificity & Preparation: This antibody recognizes the AT-1A isoform of the Angiotensin II type 1 receptor in rat, and does not recognize isoform AT-1B or AT-2. The antisera was generated in rabbits by immunization with the peptide PSDNMSSSAKKPASC (341-355) conjugated to keyhole limpet hemocyanin (KLH). Antisera was then affinity-purified by sequential passage through two affinity columns cross-linked with the carboxy terminal sequences of the rat AT-1A and AT-1B receptors. The AT-1A sequence was the same as that used for antibody production and the AT-1B peptide corresponded to amino acids 346-359. The antisera was retained by the first, but not the second column indicating recognition of the AT-1A receptor. Adsorption of the sera was also conducted by addition of AT-1A peptide (100µg/ml) to a 1:600 dilution of the antisera.

Usage: Applications include immunolabeling (1:500).

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Specificity & Preparation: This antibody recognizes the AT-1B isoform of the Angiotensin II type 1 receptor in rat, and does not recognize isoform AT-1A or AT-2. The antisera was generated in rabbits by immunization with the peptide SSSAKKSASFFEVE (346-359) conjugated to keyhole limpet hemocyanin (KLH). Antisera was then affinity-purified by sequential passage through two affinity columns cross-linked with the carboxy terminal sequences of the rat AT-1B and AT-1A receptors. The AT-1B sequence was the same as that used for antibody production and the AT-1A peptide corresponded to amino acids 341-355. The antisera was retained by the first, but not the second column indicating recognition of the AT-1B receptor.

Usage: Applications include immunolabeling (1:500).

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Specificity & Preparation: This antibody recognizes isoforms AT-1A and AT-1B of the Angiotensin II type 1 receptor (AT-1r) in rat. The antisera was generated in rabbits by immunization with the peptide PSDNMSSSAKKPASC (amino acids 341-355 of AT-1A) or with SSSAKKSASFFEVE (amino acids 346-359 of AT-1B) conjugated to keyhole limpet hemocyanin (KLH). Antisera was then affinity-purified by passage through two affinity columns, one cross-linked to AT-1A and the other with AT-1B, resulting in specificity to the sequence PSDNMSSSAKKPASCFEVE (341-359).

Usage: Applications include immunohistochemistry (immunoperoxidase electron microscopy, 1:500).

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Specificity & Preparation: This antibody recognizes the Angiotensin II type 2 receptor (AT-2) in rat. The antisera was generated in rabbits by immunization with the peptide CRKSSSLREMETFVS (349-363) conjugated to keyhole limpet hemocyanin (KLH). Antisera was then affinity-purified by passage through an affinity column cross-linked with the same peptide used for immunization.

Usage: Applications include immunolabeling (1:500).

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Specificity & Preparation: Recognizes only the phophophorylated S6 peptide product of S6K, does not cross-react with the non-phosphorylated S6 peptide substrate. Affinity-purified with phospho-peptide KRRRLApSLR on Agarose and affinity-adsorbed with non-phosphorylated S6 peptide. Conjugated to horse radish peroxidase (HRP) via reductive amination.

Usage: ELISA (0.5 µg/mL), Western Blot (2 µg/mL), immunoprecipitation (10 µg/sample).

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Specificity & Preparation: Recognizes only the phophophorylated S6 peptide product of S6K, does not cross-react with the non-phosphorylated S6 peptide substrate. Affinity-purified with phospho-peptide KRRRLApSLR on Agarose and affinity-adsorbed with non-phosphorylated S6 peptide.

Usage: Western Blot, ELISA, and non-radioactive S6K kinase assay.

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Specificity & Preparation: Recognizes ABC A1 protein in human and mouse.

Usage: ELISA (3-6 µg/ml), Western Blot (1:1,000-1:2,000), immunoprecipitation, and immunohistochemistry staining. Working concentration depends on end userís protocol.

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Specificity & Preparation: This antibody recognizes mammalian BAM 12P. The immunogen is a synthetic replicate of BAM 12P. The antibody is routinely tested by dot blot.

Usage: Applications include immunoblotting (1:4,000), immunohistochemistry (frozen fixed sections; 1:20 to 1:200), and radioimmunoassay (1:4,000).

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Specificity & Preparation: This antibody recognizes mammalian BAM 22P. The immunogen is synthetic BAM 22P. The antibody is routinely tested by dot blot.

Usage: Applications include immunoblotting (1:10,000), immunohistochemistry (frozen fixed sections; final dilution 1:200), and radioimmunoassay (1:400,000).

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Specificity & Preparation: This IgG1 k antibody recognizes the group-A epitope of the human B-cell antigen, CD22. Clone UV22-2 reacts exclusively with cells of normal human B-cell lineage. Daudi cells from a Burkittís lymphoma were used as immunogen. This antibody is routinely tested by flow cytometry.

Usage: Applications include flow cytometry (0.5 µg per 1e6 cells), indirect immuofluorescence (0.001-10µg/ml / 1.5e6 cells), radiolabeling and immunoprecipitation, radioimmunoassay, and immunoperoxidase staining (human tissues).

Also available as a targeted toxin (Cat #IT-37).

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Specificity & Preparation: This antibody recognizes the human Interleukin-2 (IL-2) receptor. Cultured human T cells were used as immunogen. This antibody was produced in tissue culture supernatants. The antibody is routinely tested by flow cytometry.

Usage: Applications include cytotoxic killer cell activity (50 µl culture supernatant), proliferation inhibition, and flow cytometry (1:10,000).

Also available as a targeted toxin (Cat #IT-24).

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Specificity & Preparation: This antibody recognizes the murine interleukin-2 (CD25) receptor. Cultured mouse B6.1 cytotoxic T-cells were used as immunogen. This antibody was produced in tissue culture supernatants.

Usage: Applications include proliferation inhibition (0.01-1.0 µg/ml), flow cytometry (2 µg), and immunoprecipitation.

Also available as a targeted toxin (Cat #IT-29).

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Specificity & Preparation: This antibody is generated at ATS using a 22 amino acid peptide from porcine ChAT (GLF SSY RLP GHT QDT LVA QKSS) as immunogen coupled to KLH and injected in rabbits. The immunogen has 95% homology with human, rat and mouse ChAT sequences, and it has about 90% homology to the chicken sequence. The antibody is expected to cross-react with rat, mouse, human, rabbit, guinea pig and porcine ChAT protein. The antibody is routinely tested by western blot analysis.

Usage: Applications include immunohistochemistry (1:2,000-4,000), immunocytochemistry and immunoblotting (western 1:1,000-2,000).

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Specificity & Preparation: This antibody is generated at ATS using a 22 amino acid peptide from porcine ChAT (GLF SSY RLP GHT QDT LVA QKSS) as immunogen coupled to KLH and injected in rabbits. The immunogen has 95% homology with human, rat and mouse ChAT sequences, and it has about 90% homology to the chicken sequence. The antibody is expected to cross-react with rat, mouse, human, rabbit, guinea pig and porcine ChAT protein. The antibody is routinely tested by western blot.

Usage: Applications include immunohistochemistry (1:2,000-4,000), immunocytochemistry and immunoblotting (western 1:1,000-2,000).

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Specificity & Preparation: This antibody recognizes human and rat corticotropin-releasing hormone/factor. The immunogen was raised in rabbits against the synthetic fragment of rat CRF (1-41). The antibody is routinely tested by dot blot.

Usage: Applications include radioimmunoassay (1:600,000), affinity chromatography (0.64 mg/ml of column), immunoblotting (1:10,000), immunostaining (1:200-1:600) and immunocytochemistry (1:1,000).

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Specificity & Preparation: This antibody recognizes the aminoterminal extracellular domain of the Corticotropin Releasing Factor receptor 2 (CRFr2) in rat. Mouse CRFr2 sequence is identical to rat CRFr2. Human and dog are 94% homologus with the Rat CRFr2 sequence. This antibody was produced in rabbit by immunization with an amino terminus peptide CHRH2 conjugated to BSA. This antibody is routinely tested by immunoblotting and flow cytometry.

Usage: Applications include immunoblotting (1:1,000), immunohistochemistry (fresh cells, 1:100), and ELISA (1:1,000-1:1,000,000).

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Specificity & Preparation: This antibody targets CYP1A2 in homo sapiens. The target antigen is human cytochrome P450. The immunogen was rat cytochrome P450 proteins in BALB-c mouse.

Usage: Applications include immunoblotting (western 1/10 dilution) and immunohistochemistry (1/5 dilution, antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6).

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Specificity & Preparation: This antibody targets CYP26A1 in homo sapiens. The target antigen is cytochrome P450 26 (EC 1.14.-.-) (Retinoic acid-metabolizing cytochrome) (P450 retinoic acid-inactivating 1) (P450RAI) (hP450RAI) (Retinoic acid 4-hydroxylase). This antibody may display some cross reactivity with P450 CYP3A43 in western blotting. The immunogen was ovalbumin-conjugated synthetic peptide PARFTHFHGE (c-terminal sequence) in BALB-c mouse.

Usage: Applications include ELISA (undiluted, titer 1/1000), immunoblotting (western undiluted), and immunohistochemistry (1/5 dilution, antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0).

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Specificity & Preparation: This antibody targets CYP2A6, CYP2A7, CYP2A13 and CYP2B6 in homo sapiens (predicted also macaca mulatta). The target antigen is CYP450 2A6 (EC 1.14.14.1) (CYPIIA6) (Coumarin 7-hydroxylase) (IIA3) (CYP2A3) (P450 (I)). The immunogen was ovalbumin-conjugated synthetic peptide RNYTMSFLPR in BALB-c mouse. CYP2A6, CYP2A7 and CYP2A13 have the same c-terminal sequence. (C-terminal sequence identical in human and rhesus macaque.)

Usage: Applications include ELISA (undiluted, titer 1/1000), immunoblotting (western 1/10 dilution), and immunohistochemistry (1/5 dilution, antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0).

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Specificity & Preparation: This antibody targets CYP3A5 in homo sapiens. The target antigen is cytochrome P450 3A5 (EC 1.14.14.1) (niphedipine oxidase), (CYPIIIA5) (P450-PCN3) (HLp2). The immunogen was ovalbumin-conjugated synthetic peptide DSRDGTLSGE (c-terminal sequence) in BALB-c mouse.

Usage: Applications include ELISA (undiluted, titer 1/16), immunoblotting (western 1/10 dilution), and immunohistochemistry (1/5 dilution, antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0).

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Specificity & Preparation: This antibody targets CYP3A7 in homo sapiens. The target antigen is cytochrome P450 3A7 (EC 1.14.14.1) (CYPIIIA7) (P450-HFLA). The immunogen was ovalbumin-conjugated synthetic peptide ESRDETVSGA (c-terminal sequence) in BALB-c mouse.

Usage: Applications include ELISA (undiluted, titer 1/1000), immunoblotting (western 1/10 dilution), and immunohistochemistry (1/5 dilution, antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0).

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Specificity & Preparation: This antibody targets CYP P450 4F11 in homo sapiens. The target antigen is cytochrome P450 4F11 (EC 1.14.14.1) (CYPIVF11). The immunogen was ovalbumin-conjugated synthetic peptide RVEPLGANSQ (c-terminal sequence) in BALB-c mouse.

Usage: Applications include ELISA (undiluted, titer 1/1000), immunoblotting (western 1/10 dilution), and immunohistochemistry (1/5 dilution, antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0).

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Specificity & Preparation: This antibody recognizes the second extracellular loop of the dopamine transporter (DAT-ECD) in rat and human. It was produced in rat by immunization with a GST-DAT-loop fusion protein, then construction of a hybridoma with the murine nonsecreting myeloma cell line Sp2/0. The second extracellular loop, consisting of amino acids 180-218, was used to construct the fusion protein.

Usage: Applications include immunocytochemistry (1-1.5 µg/ml, rat), immunohistochemistry (1:500, human), and immunoblotting (ammonium sulfate precipitated culture supernatant 1:500, rat).

Also available as a targeted toxin (Cat #IT-25).

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Specificity & Preparation: This antibody recognizes the N-terminus of the dopamine transporter (DAT-NT) in rat and human. It was produced in rat by immunization with a GST-DAT-NT fusion protein, then construction of a hybridoma with the murine nonsecreting myeloma cell line Sp2/0. The N-terminus, consisting of amino acids 1-66, was used to construct the fusion protein.

Usage: Applications include immunocytochemistry (culture supernatant 1:100, rat), immunohistochemistry (1:500, human), and immunoblotting (ammonium sulfate precipitated culture supernatant 1:500, rat).

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Specificity & Preparation: Recognizes over-expressed proteins containing† DYKDDDDK epitope tag fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells.

Usage: Western Blot (1:1000), immunoprecipitation and immunostaining (1:200).

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Specificity & Preparation: This antibody recognizes mammalian beta-endorphin. Synthetic beta-endorphin was used as immunogen. The antibody is routinely tested by dot blot. If used in western blot, this antibody may recognize the parent molecules of beta-endorphin. See cited references for details.

Usage: Applications include immunoblotting (1:5,000) and radioimmunoassay (1:28,000).

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Specificity & Preparation: This antibody recognizes mammalian [Leu5]Enkephalin. The immunogen was raised in rabbits against rat [Leu5]Enkephalin. The antibody is routinely tested by dot blot.

Usage: Applications include immunoblotting (1:1,000) and radioimmunoassay (1:5,000).

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Specificity & Preparation: Recognizes the active form of ERKís only, not the inactive. Affinity-purified with phospho-peptide (pT185/pY187) followed by immunoaffinity adsorption with non-phosphopeptide.† Conjugated to horse radish peroxidase (HRP) via reductive amination.

Usage: ELISA (0.25 µg/ml) and Western Blot (0.5 µg/ml).

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Specificity & Preparation: This antibody recognizes FGF-2 in mammals. The 1-24 synthetic fragment of bovine FGF-2 was used as immunogen. The antibody is routinely tested by dot blot.

Usage: Applications include immunoblotting (1:1,000) and immunohistochemistry (paraffin; 2.5 µg/ml).

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Specificity & Preparation: This antibody recognizes FGF-2 in rat. This antibody was made using the 1-23 synthetic fragment of rat FGF-2 (1-46) as immunogen. The antibody is routinely tested by immunoblotting.

Usage: Applications include immunoblotting, immunohistochemistry (fixed tissue 1:2,000-1:8,000; paraffin).

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Specificity & Preparation: This antibody is a mouse monoclonal antibody, affinity purified from mouse ascites fluid. Anti-DYKDDDDK (Flag) antibody recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged fusion protein. This antibody is routinely tested by western blot with ECL showing sensitivity with1-5 ng of DYKDDDDK-tagged fusion proteins.

Usage: Applications include immunoblotting (western blot with ECL 1:1,000-5,000; dot blot), immunoprecipitation, immunostaining (1:1,000-2,000), and ELISA.

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Specificity & Preparation: This antibody is specific for the 65-kDa isoform of glutamic acid decarboxylase (GAD65), targeting the IDDM-E2 region (amino acids 451-570), and does not bind the second isoform, GAD67. To create this cell line, peripheral blood B cells were obtained from a donor who tested positive for GAD65 autoantibodies and were immortalized with Epstein-Barr virus.

Usage: Applications include immunoblotting (Western, antibody supernatant 1:100), immunoprecipitation (antibody supernatant 1:5,000-1:10,000), immunohistochemistry, and protein footprinting (10 µl serum).

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Specificity & Preparation: This antibody is specific for the 65-kDa isoform of glutamic acid decarboxylase (GAD65), targeting the IDDM-E2 region (amino acids 451-570), and does not bind GAD67. To create this cell line, peripheral blood B cells were obtained from a donor who tested positive for GAD65 autoantibodies and were immortalized with Epstein-Barr virus.

Usage: Applications include immunoprecipitation (antibody supernatant 1:5,000-1:10,000), immunohistochemistry, and protein footprinting (10 µl serum). This antibody does not work in Western blotting.

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Specificity & Preparation: This antibody is a mouse monoclonal antibody, affinity purified from mouse ascites fluid. Anti-GAPDH recognizes native and denatured forms of GAPDH in human, mouse, rat, rabbit, bacteria, etc. This antibody is routinely tested by western blot.

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GAPDH Mouse Monoclonal (Cat. #AB-267, 100 µg)

Specificity & Preparation: Recognizes native and denatured forms of GAPDH from human, mouse, rat and rabbit. Recombinant GAPDH produced in bacteria (BL-21), insect cells (SF9), and yeast (Saccharomyces cerevisiae) is also recognized. GAPDH from other species may also be detectable.

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GAT-1 Rabbit Polyclonal (Cat. #AB-N37, 50 µg)

Specificity & Preparation: This antibody recognizes the -aminobutyric acid (GABA)-1 transporter, GAT-1, in rat. The antibody is an affinity-purified rabbit polyclonal. The peptide used as an antigen has 100% sequence homology between rat, human, mouse, and bovine GAT-1.

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GFP Mouse Monoclonal (Cat. #AB-268, 100 µg)

Specificity & Preparation: Recognizes native and denatured forms of GFP and its variants EGFP, YFP, EYFP, and CFP. This antibody is tested by western blot with ECL showing sensitivity with 1-10 ng of purified GFP or GFP fusion proteins.

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Growth Hormone Releasing Factor Rabbit Polyclonal - affinity-purified rat (Cat. #AB-16ap, 50 µg)

Specificity & Preparation: This antibody recognizes the growth hormone releasing hormone/factor in rat. Synthetic hpGRF 1-40 was used as immunogen. The antibody was purified from polyclonal antiserum using a CRF affinity column. The antibody is routinely tested by immunoblotting.

Usage: Applications include immunoblotting (1:1,000-1,10:000) and ELISA (1:1,000-1:10,000).

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Specificity & Preparation: This antibody recognizes the human growth hormone releasing hormone/factor. Synthetic hpGRH1-40 was used as immunogen. The antibody is routinely tested by dot blot.

Usage: Applications include immunoblotting (1:1,000), immunostaining (1:200 to 1:600), immunohistochemistry (fresh or frozen non-fixed sections; 1:800) and radioimmunoassay.

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Specificity & Preparation: This antibody recognizes the growth hormone releasing hormone/factor in rat. Synthetic hpGRF 1-40 was used as immunogen. The antibody is routinely tested by immunoblotting.

Usage: Applications include immunoblotting (1:1,000) and ELISA (1:500).

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Specificity & Preparation: Detects over-expressed glutathione-transferase (GST) fusion proteins.

Usage: Western Blot (1:1000), immunoprecipitation and immunostaining (1:200).

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Specificity & Preparation: Recognizes HA-tagged proteins over-expressed in cells, including both amino- or carboxy-termini of targeted proteins in transfected mammalian cells.

Usage: Western Blot (1:1000), immunoprecipitation and immunostaining (1:200).

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Specificity & Preparation: Recognizes His-tagged recombinant proteins or His-tagged proteins over-expressed in cells.

Usage: Western Blot (1:1000), immunoprecipitation and immunostaining (1:200). This antibody has also been used in ELISA and dot-blots; the dilutions for these applications are assay dependent.

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Specificity & Preparation: Recognizes Histone H3 in human and mouse. Affinity-purified with Histone H3 peptide. Conjugated to horse radish peroxidase (HRP) via reductive amination.

Usage: ELISA (1:1000) and Western Blot (1:250-1000).

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Specificity & Preparation: Recognizes Histone H3 in human and mouse. Affinity-purified with Histone H3 peptide.

Usage: ELISA (1:1000) and Western Blot (1:250-1000).

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Specificity & Preparation: Recognizes Histone H4 in human, rat, bovine, mouse, and rabbit. Does not cross-react with histone H1, H2 and H3. Affinity-purified with Histone H4 peptide on Agarose.

Usage: ELISA, Western Blot, and immunoprecipitation.

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Specificity & Preparation: This antibody recognizes the 70 kD human albumin. This antibody is affinity-purified with human serum albumin (HSA) on CnBr-activated Sepharose 4 Fast Flow followed by affinity adsorption with human IgG. This affinity-purified anti-HSA is routinely tested by Western Blot.

Usage: Applications include ELISA and immunobloting (Western Blot 1:10,000, ATS in-house).

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Specificity & Preparation: Recognizes 70 kD human albumin, does not cross-react with bovine serum albumin or human IgG. Affinity-purified with†HSA on Agarose followed by affinity adsorption with bovine serum albumin (BSA) and human IgG. Conjugated to horse radish peroxidase (HRP) via reductive amination.

Usage: ELISA and Western Blot.

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Specificity & Preparation: Recognizes 70 kD human albumin, cross-reacts with bovine serum albumin, but not with human IgG. Affinity-purified with†HSA on Agarose followed by affinity adsorption with†human IgG.

Usage: ELISA, Western Blot, immunoprecipitation, and urinary protein capture assay.

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Specificity & Preparation: Recognizes JNK. The recognition sequence is identical in JNK3, JNK2, and JNK1, in human, mouse, and rat. Affinity-purified with peptide-affinity chromatography. Conjugated to horse radish peroxidase (HRP) via reductive amination.

Usage: ELISA (1:1000) and Western Blot (1:250-1:1000).

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Specificity & Preparation: Recognizes JNK. The recognition sequence is identical in JNK3, JNK2, and JNK1, in human, mouse, and rat. Affinity-purified with peptide-affinity chromatography. Conjugated to horse radish peroxidase (HRP) via reductive amination.

Usage: ELISA (1:1000) and Western Blot (1:250-1:500).

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Specificity & Preparation: Recognizes human and bovine LPL.

Usage: Western Blot (1:100-1:250) and ELISA (1:2000~5000).

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Specificity & Preparation: This antibody recognizes mammalian Luteinizing Hormone Releasing Hormone (LHRH). Ovine-LHRH was used as immunogen. This antibody is routinely tested by dot blot.

Usage: Applications include immunostaining (1:200 to 1:600), immunoblotting (1:1,000) and radioimmunoassay.

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Specificity & Preparation: This antibody recognizes rat Mac-1 (CD11b). The hybridoma was formed by the fusion of mouse myeloma line NS-1 with splenocytes from mice immunized with rat neutrophils. The antibody is routinely tested by flow cytometry.

Usage: Applications include flow cytometry (saturating concentration).

Also available as a targeted toxin (Cat #IT-33).

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Specificity & Preparation: This antibody recognizes human and mouse Mac-1 (CD11b). The hybridoma was formed by the fusion of mouse myeloma NS1 cells with spleen cells from rats immunized with B10 mouse spleen cells enriched for T-lymphocytes. The antibody is routinely tested by flow cytometry.

Usage: Applications include immunoprecipitation, flow cytometry (50 µl), cytotoxicity assay (ED50=16 pM), and binding assay (5 and 50 µl of culture supernatant.

Also available as a targeted toxin (Cat #IT-06).

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Specificity & Preparation: Recognizes 20 kD bovine myelin basic proteins (MBP). May cross-react with other isoforms of other mammalian myelin basic proteins as the affinity binding-sequence is identical. Affinity-purified with pan MBP peptide (PRTPPPSQGKGRGL) on Agarose.

Usage: ELISA, Western Blot, and immunoprecipitation.

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Specificity & Preparation: This antibody recognizes the metabotrophic glutamate receptor 2, but not metabotrophic glutamate receptor 3, in rat and mouse. The antibody was made against a GST-fusion with a 47-amino acid sequence against the C-terminal portion of mGluR2.

Usage: Applications include immunoblotting (western, 1 µg/ml), immunohistochemistry (1 µg/ml), immunostaining (1 µg/ml), and immunofluorescence (1 µg/ml).

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Specificity & Preparation: Recognizes proteins methylated on lysine residues (mono and di-methylysine). Does not cross-react with acetylated proteins. Cross-reactivity with tri-methyllysine is not tested. Affinity-purified using Ne-mnomethyllysine and dimehyllysine on Agarose as affinity matrix. Conjugated to d-Biotin via acylation.

Usage: ELISA (1:1000) and Western Blot (1:1000).

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Specificity & Preparation: Recognizes proteins methylated on lysine residues (mono and di-methylysine). Does not cross-react with acetylated proteins. Affinity-purified using N-methyl (epsilon amino group) lysine on Agarose as affinity matrix. Conjugated to horse radish peroxidase (HRP) via reductive amination.

Usage: ELISA, Western Blot, and immunohistochemistry.

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Specificity & Preparation: Recognizes proteins or peptides methylated on lysine residues (mono, di-methylysine). Does not cross-react with acetylated proteins. Cross-reactivity with tri-methyllysine is not tested. Immunoaffinity-purified with epsilon N-methyl lysine on Agarose.

Usage: ELISA (1:2000), Western Blot (1:250-1000), and immunoprecipitation (10 µg/mg crude protein).

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Specificity & Preparation: This antibody targets human matrix metalloproteinase 1 (MMP-1) in homo sapiens. The immunogen was ovalbumin-conjugated synthetic peptide CSSFGFPRTVKH (c-terminal sequence specific to human) in BALB-c mouse.

Usage: Applications include immunoblotting (western undiluted) and immunohistochemistry (undiluted, antigen retrieval: microwave 10 min @ 950W in 10 mM citrate buffer, pH 6.0).

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Specificity & Preparation: This antibody targets human matrix metalloproteinase 2 (MMP-2) in homo sapiens, oryctolagus cuniculus. The immunogen was ovalbumin-conjugated synthetic peptideTSLGLPPDVQRVD (c-terminal sequence common to human and rabbit) in BALB-c mouse.

Usage: Applications include ELISA (undiluted), immunoblotting (western undiluted), and immunohistochemistry (undiluted, antigen retrieval: microwave 5 min @ 950W in 10 mM citrate buffer, pH 6.0).

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Specificity & Preparation: This antibody targets human matrix metalloproteinase 3 (MMP-3) in homo sapiens. The immunogen was ovalbumin-conjugated synthetic peptide CKSLRKLEPELH (c-terminal sequence specific to human) in BALB-c mouse.

Usage: Applications include ELISA (undiluted), immunoblotting (western undiluted), and immunohistochemistry (undiluted, antigen retrieval: microwave 5 min @ 950W in 10 mM citrate buffer, pH 6.0).

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Specificity & Preparation: This antibody targets human matrix metalloproteinase 9 (MMP-9) in homo sapiens. The immunogen was ovalbumin-conjugated synthetic peptide KLGLGADVAQVT (c-terminal sequence specific to human) in BALB-c mouse.

Usage: Applications include immunoblotting (western undiluted) and immunohistochemistry (undiluted, antigen retrieval: microwave 10 min @ 950W in 10 mM citrate buffer, pH 6.0).

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Specificity & Preparation: This antibody recognizes mammalian gamma-1-MSH. Synthetic gamma-1-MSH was used as immunogen. The antibody is routinely tested by dot blot.

Usage: Applications include immunocytochemistry (1:1,000-1:20,000), immunoblotting (1:10,000) and radioimmunoassay (1:500,000).

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Specificity & Preparation: Recognizes over-expressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells.

Usage: Western Blot (1:1000), immunoprecipitation and immunostaining (1:200).

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Specificity & Preparation: This antibody recognizes the p75NTR (low affinity neurotrophin receptor) in human, primate, rabbit, sheep, dog, cat, and pig. It was derived from immunization of mice with WM245 melanoma cells. The antibody is routinely tested by flow cytometry.

Usage: Applications include flow cytometry (1:100), immunoprecipitation, immunohistochemistry (frozen), electron microscopy (1:200), immunocytochemistry (10 ng/ml), and radioimmunoassay.

Also available as a targeted toxin (Cat #IT-15).

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Specificity & Preparation: This antibody recognizes p75NTR in mouse. The antisera was developed in rabbit using an extracellular fragment from the mouse p75 receptor (amino acids 43-161). The antibody was affinity-purified using the extracellular domain of p75. The antibody is routinely tested by flow cytometry.

Usage: Applications include immunohistochemistry (frozen or paraffin-embedded cells and tissue; 1:150), immunoprecipitation, immunoblotting (1:2,000), flow cytometry (1:1,000), and blocking the function of nerve growth factor receptor (1:1,000).

Also available as a targeted toxin (Cat #IT-16). Also available as a fluorescent conjugate (Cat #FL-05, FL-06, FL-09).

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Specificity & Preparation: This antibody recognizes the p75NTR in mouse. The antisera was developed in rabbit using an extracellular fragment from the mouse p75 receptor (amino acids 43-161). The antibody is routinely tested by flow cytometry.

Usage: Applications include immunohistochemistry (frozen or paraffin-embedded cells and tissue; 1:150), immunoprecipitation, immunoblotting (1:2,000), flow cytometry (1:1,000), and blocking the function of nerve growth factor receptor (1:1,000).

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Specificity & Preparation: This antibody binds to nicotinic acetylcholine receptors (nAChR) in several species, including human and rat. The antibody was originally raised against the electric organ of Electrophorus electricus and produces experimental autoimmune myasthenia graves. mAb 35 cross-reacts with muscle-type and some neuronal nAChRs. It reacts with a single epitope in a1, a3, and a5 subunits of nAChR.

Usage: Applications include immunohistochemistry (1:3000), in vivo (20 µg), immunocytochemistry (10 nM), upregulation of myocytes in vitro (50 µg/ml), and receptor affinity purification.

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Specificity & Preparation: This antibody recognizes the NK-1 receptor in rat, dog, and human. This antibody was developed in rabbit using a synthetic peptide corresponding to an amino acid sequence of dog NK-1 receptor conjugated to bovine thyroglobuline with glutaraldehyde. The peptide sequence has a high degree of homology to other species such as human, mouse, rat and guinea pig. The antibody is routinely tested by immunohistochemistry and flow cytometry.

Usage: Applications include immunohistochemistry (peroxidase substrate 1-3 µg/ml and fluorescent 1-3 µg/ml), flow cytometry (3-10 µg/ml), immunoblotting (western blot analysis 1-3 µg/ml ), and ELISA (1:1,000,000).

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Specificity & Preparation: This antibody recognizes the NK-1 receptor in rat, mouse, and guinea pig. The NK-1 receptor histochemical antisera was developed in rabbit using a synthetic peptide corresponding to a 15-amino acid sequence (393-407) of the COOH-terminus of the rat NK-1 receptor conjugated to bovine thyroglobulin with glutaraldehyde.

Usage: Applications include immunohistochemistry in brain and spinal cord of rat and mouse (1:500 to 1:5,000) and immunoblotting (1:1,000).

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Specificity & Preparation: This antibody targets isoenzyme neuron-specific enolase (NSE) in homo sapiens, mus musculus, gallus gallus. The immunogen was ovalbumin-conjugated synthetic peptide corresponding to human NSE amino acid sequence: NSE-P1 (aaís 416-433 - LGDEARFAGHNFRNPSVL), NSE-P2 (aaís 271-285 - TGDQLGALYQDFVRD) in BALB-c mouse.

Usage: Applications include ELISA, immunoblotting (western undiluted), and immunohistochemistry.

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Specificity & Preparation: Reacts with ochratoxin A and C, slightly cross-reacts with ochratoxin B, and does not cross-react with ochratoxin. Affinity-purified with Ochratoxin A on agarose.

Usage: ELISA.

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Specificity & Preparation: This antibody recognizes cells that express Thy-1.1 in rat, mouse, rabbit or guinea pig. Note: Antibody reactivity and working conditions may vary between species. Anti-OX7 was created as a mouse monoclonal generated to rat Thy-1.1 (CD90). The antibody is routinely tested by flow cytometry.

Usage: Applications include immunohistochemistry (frozen; 1:2), immunofluorescence, flow cytometry, radioimmunoassay (1:10). Causes glomerulosclerosis when injected intravenously in rats and mice.

Also available as a targeted toxin (Cat #IT-02).

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Specificity & Preparation: Recognizes p33 ING1 of human and mouse. Does not cross-react with other ING family. Affinity-purified with the p33 ING c-terminal peptide on Agarose. Conjugated to d-Biotin via acylation of primary amine with active ester of biotin (NHS-activated d-biotin).

Usage: ELISA (0.25 µg/mL) and Western Blot (1 µg/mL).

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Specificity & Preparation: Recognizes p33 ING1 of human and mouse. Does not cross-react with other ING family. Affinity-purified with the p33 ING c-terminal peptide on Agarose. Conjugated to horse radish peroxidase (HRP) via reductive amination.

Usage: ELISA (0.25 µg/mL) and Western Blot (1 µg/mL).

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Specificity & Preparation: Recognizes p33 ING1 of human and mouse. Does not cross-react with other ING family. Affinity-purified with the p33 ING c-terminal peptide on Agarose.

Usage: ELISA (0.5 µg/mL), Western Blot (1 µg/mL), and immunofluorescence (1:50).

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Specificity & Preparation: Recognizes p42 ERK2 of human rat, bovine, mouse, and rabbit. Does not cross-react with p44 ERK1. Affinity-purified with p42 ERK2 c-terminal peptide on Agarose.

Usage: ELISA (0.5 µg/mL), Western Blot (1 µg/mL), immunoprecipitation (5 µg/sample).

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Specificity & Preparation: Recognizes p42 MAPK (ERK2), does not cross-react with p44 MAPK (ERK1). Affinity-purified with p42 ERK2 c-terminal peptide on Agarose. Conjugated to horse radish peroxidase (HRP) via reductive amination.

Usage: ELISA (0.25 µg/mL) and Western Blot (1 µg/mL).

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Specificity & Preparation: Recognizes p44 ERK1 of human rat , bovine, mouse, rabbit, does not cross-react with p42 ERK2. Affinity-purified with p44 ERK1 c-terminal peptide on Agarose.

Usage: ELISA (0.5 µg/mL), Western Blot (1 µg/mL), immunoprecipitation (5 µg/sample).

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Specificity & Preparation: Recognizes p44 MAPK (ERK1), does not cross-react with p42 MAPK (ERK2). Affinity-purified with p44 ERK1 c-terminal peptide on Agarose. Conjugated to d-Biotin via acylation of primary amine with active ester of biotin (NHS-activated d-biotin).

Usage: ELISA (0.25 µg/mL) and Western Blot (0.5 µg/mL).

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Specificity & Preparation: Recognizes p44 MAPK (ERK1), does not cross-react with p42 MAPK (ERK2). Affinity-purified with p44 ERK1 c-terminal peptide on Agarose. Conjugated to horse radish peroxidase (HRP) via reductive amination.

Usage: ELISA (0.25 µg/mL) and Western Blot (1 µg/mL).

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Specificity & Preparation: Recognizes only phosphor p53 in human, not mouse. Affinity-purified with peptide-affinity chromatography.

Usage: ELISA (1:2500) and Western Blot (1:2500).

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Specificity & Preparation: Recognizes human p53 in cell and tissue. Affinity-purified with peptide-affinity chromatography. Conjugated to horse radish peroxidase (HRP) via reductive amination.

Usage: ELISA (1:2500) and Western Blot (1:2500).

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Specificity & Preparation: Recognizes human p53 in cell and tissue. Affinity-purified with peptide-affinity chromatography.

Usage: ELISA (1:2500) and Western Blot (1:500-2500).

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Specificity & Preparation: Recognizes p70 S6K of human, mouse, and rat. Does not cross react with p90 RSK. Affinity-purified with peptide (ISKRPEHLRMNL) on Agarose. Conjugated to d-Biotin via acylation of primary amine with active ester of biotin (NHS-activated d-biotin).

Usage: ELISA and Western Blot.

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Specificity & Preparation: Recognizes p70 S6K of human, mouse, rat. Does not cross-react with p90 RSK. Affinity-purified with peptide (ISKRPEHLRMNL) on Agarose. Conjugated to horse radish peroxidase (HRP) via reductive amination.

Usage: ELISA and Western Blot.

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Specificity & Preparation: Recognizes p70 S6K of human, mouse, and rat. Affinity-purified with peptide (ISKRPEHLRMNL) on Agarose.

Usage: ELISA (0.5 µg/mL), Western Blot (1 µg/mL), immunoprecipitation (5 µg/sample).

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Specificity & Preparation: Recognizes p90 RSK1 of human, mouse, and rat. Does not cross-react with p70 S6K. Affinity-purified with peptide (C-LAQRRVRKLPSTTL) on Agarose. Conjugated to d-Biotin via acylation of primary amine with active ester of biotin (NHS-activated d-biotin).

Usage: ELISA (0.25 µg/mL) and Western Blot (1 µg/mL).

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Specificity & Preparation: Recognizes 90 kD RSK1, does not cross-react with p70 S6K. Affinity-purified with peptide (C-LAQRRVRKLPSTTL) on Agarose. Conjugated to horse radish peroxidase (HRP) via reductive amination.

Usage: ELISA and Western Blot.

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Specificity & Preparation: Recognizes p90 RSK1 of human, mouse, and rat. Affinity-purified with peptide (C-LAQRRVRKLPSTTL) on Agarose.

Usage: ELISA, Western Blot, and immunoprecipitation.

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Specificity & Preparation: Recognizes only the phosphorylated Crebtide product of PKA and PKC in a non-radioactive kinase assay, does not cross-react with the non-phosphorylated Crebtide. Affinity-purified with KRREILSRRPpSYR on Agarose followed by affinity adsorption with non-phosphorylated Crebtide. Biotinylated with NHS-activated biotin and G25 purification.

Usage: ELISA, Western Blot, and non-radioactive PKA and PKC kinase assay.

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Specificity & Preparation: Recognizes only the phosphorylated Crebtide product of PKA and PKC in a non-radioactive kinase assay, does not cross-react with the non-phosphorylated Crebtide. Affinity-purified with KRREILSRRPpSYR on Agarose followed by affinity adsorption with non-phosphorylated Crebtide. Conjugated to horse radish peroxidase (HRP) via reductive amination.

Usage: ELISA (1:1000) and Western Blot (1:1000).

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Specificity & Preparation: Recognizes only the phosphorylated Crebtide product of PKA and PKC in a non-radioactive kinase assay, does not cross-react with the non-phosphorylated Crebtide. Affinity-purified with KRREILSRRPpSYR on Agarose followed by affinity adsorption with non-phosphorylated Crebtide.

Usage: ELISA, Western Blot, and non-radioactive PKA and PKC kinase assay.

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Specificity & Preparation: Recognizes only the phosphorylated Crosstide, the product of SGK, PKB in a non-radioactive kinase assay, does not cross-react with the non-phosphorylated Crosstide. Affinity-purified with PRAApTF on Agarose followed by affinity adsorption with non-phosphorylated Crosstide. Conjugated to horse radish peroxidase (HRP) via reductive amination.

Usage: ELISA (1:1000) and Western Blot (1:1000).

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Specificity & Preparation: Recognizes only the phophophorylated Crosstide, the product of SGK, PKB in a non-radioactive kinase assay, does not cross-react with the non-phosphorylated Crosstide. Affinity-purified with PRAApTF on Agarose followed by affinity adsorption with non-phosphorylated Crosstide.

Usage: ELISA, Western Blot, and non-radioactive AKT and SGK kinase assay.

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Specificity & Preparation: Pan-specific for phosphoserine, does not cross-react with phosphotyrosine, but slightly reacts with phopsphothreonine. Affinity-purified with phosphoserine on Agarose. Conjugated to d-Biotin via acylation of primary amine with active ester of biotin (NHS-activated d-biotin).

Usage: ELISA (1:1000) and Western Blot (1:1000).

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Specificity & Preparation: Recognizes proteins phosphorylated on serine residues. Does not cross-react with phosphotyrosine. Affinity-purified with phosphoserine on Agarose. Conjugated to horse radish peroxidase (HRP) via reductive amination.

Usage: ELISA and Western Blot.

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Specificity & Preparation: Recognizes proteins phosphorylated on serine residues. Does not cross-react with phosphotyrosine. Affinity-purified with phosphoserine on Agarose.

Usage: ELISA (1:250), Western Blot (1:250), immunoprecipitation (10 µg/mg crude protein), and immunohistochemistry (1:50).

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Specificity & Preparation: Pan-specific for phosphothreonine, slightly cross-reacts with phosphoserine, but does not cross-react with phosphotyrosine. Affinity-purified with phosphothreonine immobilized on agarose. Conjugated to d-Biotin via acylation of primary amine with active ester of biotin (NHS-activated d-biotin).

Usage: ELISA (1:1000) and Western Blot (1:1000).

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Specificity & Preparation: Pan-specific for phosphothreonine, slightly cross-reacts with phosphoserine, but does not cross-react with phosphotyrosine. Affinity-purified with phosphothreonine immobilized on agarose. Conjugated to horse radish peroxidase (HRP) via reductive amination.

Usage: ELISA (1:1000) and Western Blot (1:500).

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Specificity & Preparation: Recognizes proteins phosphorylated on threonine residues. Does not cross-react with phosphotyrosine. Affinity-purified with phosphothreonine on Agarose.

Usage: ELISA (0.5 µg/mL), Western Blot (2 µg/mL), immunoprecipitation (10 µg/mg sample).

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Specificity & Preparation: Pan-specific for phosphotyrosine, does not cross-react with phosphor-serine or -threonine. Affinity-purified with phosphotyrosine immobilized on agarose. Conjugated to d-Biotin via acylation of primary amine with active ester of biotin (NHS-activated d-biotin).

Usage: ELISA (1:1000) and Western Blot (1:1000).

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Specificity & Preparation: Pan-specific for phosphotyrosine, but does not cross-react with phosphor-serine or -threonine. Affinity-purified with phosphotyrosine immobilized on agarose followed by immune affinity adsorption with tyrosine on Agarose. Conjugated to horse radish peroxidase (HRP) via reductive amination.

Usage: ELISA (1:1000), Western Blot (1:1000), and Tyrosine kinase assay.

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Specificity & Preparation: Recognizes proteins and peptides phosphorylated on tyrosine residues. Does not cross-react with phosphoserine and threonine. Affinity-purified with phosphotyrosine on Agarose.

Usage: ELISA (1:1000), Western Blot (1:250), immunoprecipitation (10 µg/500 µg protein sample), immunohistochemistry (1:50), and non-radioactive tyrosine kinase assay.

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Specificity & Preparation: This antibody recognizes native and recombinant saporin. It was developed in chicken using saporin and was purified from chicken egg yolk. The antibody is routinely tested by western blot.

Usage: Applications include immunoblotting (1:1,000), ELISA (1 µg/well), and flow cytometry (1:50).

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Specificity & Preparation: This antibody recognizes saporin. Saporin was used as the immunogen. The antibody was affinity-purified against saporin attached to a CnBr-Sepharose support column. The antibody is routinely tested by Western blot.

Usage: Applications include immunoblotting (1:1,000).

Also available as a fluorescent conjugate (Cat #FL-02).

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Specificity & Preparation: This antibody recognizes saporin. The antibody was first protein-A-purified followed by affinity-purification against saporin attached to a CnBr-Sepharose support column. This antibody is routinely tested by Western Blot.

Usage: Applications include immunoblotting (Western Blot ATS in-house; 1:1,000).

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Specificity & Preparation: This antibody recognizes saporin. Saporin was used as the immunogen. The antibody is routinely tested by Western blot.

Usage: Applications include immunoblotting (1:1,000), ELISA (1:100), and immunohistochemistry (frozen, fixed sections; 1:1,000 or 1:350).

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Specificity & Preparation: This antibody recognizes saporin. Saporin was used as the immunogen. The antibody was coupled to Horseradish Peroxidase (HRP) and dialyzed against PBS. The conjugated antibody is routinely tested by western blot.

Usage: Applications include ELISA and immunoblotting.

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Specificity & Preparation: This antibody recognizes the 40-amino-acid peptide, sauvagine. Synthetic sauvagine was used as immunogen. The antibody is routinely tested by dot blot.

Usage: Applications include immunoblotting (1:1,000).

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Specificity & Preparation: Reacts with human and mouse proteins.

Usage: Western Blot, ELISA, immunoprecipitation, and immunohistochemistry.

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Specificity & Preparation: This antibody recognizes cells that express SERT in rat, human, and mouse. The immunogen is a peptide from the fourth extracellular domain of the rat SERT. This antibody was produced in tissue culture supernatants. The antibody is routinely tested by flow cytometry.

Usage: Applications include flow cytometry (1:50), immunohistochemistry (paraffin), immunostaining (1:500), immunocytochemistry.

Also available as a targeted toxin (Cat #IT-23). Peptide or Antigen also available (Cat. #PR-03).

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Specificity & Preparation: This antibody was raised against rat somatosatin receptor-1 (SSTr1) and recognizes SSTr1 in human. The somatostatin receptor antisera was developed in rabbit using a peptide corresponding to the extracellular domain conjugated to keyhole limpet hemocyanin (KLH) for immunization. Antisera was then affinity-purified with the peptide utilized for immunization. The antibody is routinely tested by immunoblotting and flow cytometry.

Usage: Applications include immunoblotting and flow cytometry (1:500).

Peptide or Antigen also available (Cat. #PR-04).

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Specificity & Preparation: This antibody was raised against rat somatostatin receptor-1 (SSTr1) and recognizes SSTr1 in human and rat. The SSTr1 monoclonal antibody was developed using a peptide corresponding to the extracellular domain of SSTr1 conjugated to keyhole limpet hemocyanin (KLH). This antibody is routinely tested by flow cytometry.

Usage: Applications include immunohistochemistry and immunocytochemistry (2-10 µg/ml), flow cytometry (2-10 µg/ml), immunoblotting (western blot 2-10 µg/ml), and ELISA (1:125,000).

Peptide or Antigen also available (Cat. #PR-04).

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Specificity & Preparation: This antibody was raised against rat somatosatin receptor-1 (SSTr1) and recognizes SSTr1 in human. The somatostatin receptor antisera was developed in rabbit using a peptide corresponding to the extracellular domain conjugated to keyhole limpet hemocyanin (KLH) for immunization. The antibody is routinely tested by immunoblotting.

Usage: Applications include ELISA (1:500) and immunoblotting.

Peptide or Antigen also available (Cat. #PR-04).

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Specificity & Preparation: This antibody was raised against rat somatosatin receptor-2 (SSTr2) and recognizes SSTr2 in human. The somatostatin receptor antisera was developed in rabbit using a peptide corresponding to the extracellular domain conjugated to keyhole limpet hemocyanin (KLH) for immunization. This antibody is routinely tested by immunoblotting.

Usage: Applications include immunoblotting and ELISA (1:500).

Peptide or Antigen also available (Cat. #PR-05).

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Specificity & Preparation: This antibody was raised against rat somatostatin receptor-4 (SSTr4) and recognizes SSTr4 in rat. The SSTr antisera was developed in rabbit using a peptide (Cat. #PR-07) corresponding to the extracellular domain conjugated to keyhole limpet hemocyanin (KLH) for immunization. Antisera was then affinity-purified with the peptide utilized for immunization. The antibody is routinely tested by immunoblotting.

Usage: Applications include immunoblotting and ELISA.

Peptide or Antigen also available (Cat. #PR-07).

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Specificity & Preparation: This antibody was raised against rat somatostatin receptor-5 (SSTr5) and recognizes SSTr5 in human and rat. The SSTr5 monoclonal antibody was developed using a peptide corresponding to the extracellular amino terminal domain of rat SSTr5 conjugated to keyhole limpet hemocyanin (KLH). This antibody is routinely tested by flow cytometry.

Usage: Applications include immunohistochemistry and immunocytochemistry (2-10 µg/ml), flow cytometry (2-10 µg/ml) and ELISA (1:500).

Peptide or Antigen also available (Cat. #PR-08).

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Specificity & Preparation: This antibody recognizes mammalian somatostatin-14. [Tyr10] somatostatin-14 was used as immunogen. The antibody is routinely tested by dot blot.

Usage: Applications include immunostaining (1:200 to 1:600) and immunoblotting (1:35,000).

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Specificity & Preparation: This antibody recognizes mammalian somatostatin-28. Somatostatin-28 (6-28) was used as immunogen. The antibody is routinely tested by dot blot.

Usage: Applications include immunostaining (1:200 to 1:600), immunoblotting (1:10,000) and radioimmunoassay (1:5,000 or 1:10,000).

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Specificity & Preparation: Recognizes stat1. Affinity-purified with peptide-affinity chromatography.

Usage: ELISA, Western Blot, and immunohistochemistry.

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Specificity & Preparation: Recognizes stat3 in mouse and human. Affinity-purified with peptide-affinity chromatography.

Usage: ELISA (1:2500), Western Blot (1:2500), and immunohistochemistry (1:100).

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Specificity & Preparation: Recognizes stat5 in human and mouse. Affinity-purified with peptide-affinity chromatography.

Usage: ELISA (1:2500), Western Blot (1:2500), and immunohistochemistry (1:100).

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Specificity & Preparation: This antibody recognizes Substance P (SP). It was developed in rabbits using Substance P conjugated to BSA as the immunogen. The antibody is routinely tested by ELISA and western blot.

Usage: Applications include ELISA (1:1,000) and immunoblotting (1:1,000).

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Specificity & Preparation: This antibody targets human tissue inhibitor of matrix metalloproteinase 1 (TIMP-1) in homo sapiens, macaca mulatta. The immunogen was ovalbumin-conjugated synthetic peptide FQALGDAADIR (sequence common to human and monkey) in BALB-c mouse.

Usage: Applications include ELISA (undiluted), immunoblotting (western undiluted), and immunohistochemistry (undiluted, antigen retrieval: microwave 7 min @ 950W in 10 mM citrate buffer, pH 6.0).

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Specificity & Preparation: This antibody targets human tissue inhibitor of matrix metalloproteinase 2 (TIMP-2) in homo sapiens, mus musculus, oryctolagus cuniculus, gallus gallus, brachydanio rerio, sus scrofa. The immunogen was ovalbumin-conjugated synthetic peptide DSGNDIYGNPI (sequence common to human, mouse, rabbit, chicken, zebrafish, pig) in BALB-c mouse.

Usage: Applications include ELISA (1/10 dilution), immunoblotting (western undiluted), and immunohistochemistry (undiluted, antigen retrieval: microwave 7 min @ 950W in 10 mM citrate buffer, pH 6.0).

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Specificity & Preparation: This antibody recognizes the human telomeric repeat binding factor 1 (TRF1). Full length recombinant human TRF1 was used as immunogen. It was produced in cell culture supernatant and contains 0.005% Thimerosal as a preservative. The antibody is routinely tested by western blot.

Usage: Applications include western blot (1:200).

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Specificity & Preparation: Recognizes proteins with trimethylation on lysine residues (N-epsilon)). Does not cross-react with acetylated proteins and mono- and dimethylated proteins. Affinity-purified from the anti-serum against chemically methylated protein antigen. Biotinylated with NHS-activated d-biotin.

Usage: ELISA (1:2000), Western Blot (1:2000), immunoprecipitation (5 µg/mg protein sample), immunofluorescent staining (5 µg/mL or 1:100).

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Specificity & Preparation: Recognizes proteins with trimethylation on lysine residues (N-epsilon)). Does not cross-react with acetylated proteins and mono- and dimethylated proteins. Affinity-purified from the anti-serum against chemically methylated protein antigen. Conjugated to horse radish peroxidase (HRP) via reductive amination.

Usage: ELISA (1:5000) and Western Blot (1:5000).

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Specificity & Preparation: Recognizes proteins with trimethylation on lysine residues (N-epsilon)). Does not cross-react with acetylated proteins and mono- and dimethylated proteins. Affinity-purified from the anti-serum against chemically methylated protein antigen.

Usage: ELISA (1:2000), Western Blot (1:1000), immunoprecipitation (5 µg/mg protein sample), immunofluorescent staining (5 µg/mL or 1:100), immunohistochemistry (1:1000).

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Specificity & Preparation: This antibody recognizes rat trkA (high affinity nerve growth factor receptor). Histochemical antisera to trkA was developed in rabbit using the extracellular fragment from rat trkA (amino acids 1-416); this product has been purified by protein A chromatography. The antibody is routinely tested by western blot.

Usage: Applications include immunohistochemistry (frozen sections; 1:1,000 or 1:10,000), immunoprecipitation (2.5 µg antibody per 30 µl Protein-A Sepharose), and immunoblotting (1 µg/ml).

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Specificity & Preparation: This antibody recognizes the extracellular domain of the human TSH receptor (TSHr). The murine monoclonal antibody A10 is specific for residues 22-35 of the TSHr. This epitope is localized at the amino terminal of the extracellular domain of the TSHr, a region that may be masked from the surface of native TSHr. It was produced in mouse by immunization with a recombinant GST-TSHr fusion protein, then construction of a hybridoma with the non-immunoglobulin-secreting myeloma cell line X63-Ag8-653. Amino acids 22-35 at the amino terminus of the TSHr were used to construct the fusion protein.

Usage: Applications include immunoblotting (Western, ascites 1:10,000), immunohistochemistry (acetone-fixed cells, ascites 1:50), immunoprecipitation, and ELISA (1:1,000-1:1,000,000 of ascites).

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Specificity & Preparation: This antibody recognizes the extracellular domain of the human TSH receptor (TSHr). The murine monoclonal antibody A7 is specific for residues 402-415 of the TSHr. This epitope is localized at the extreme carboxyl terminal of the extracellular domain of the TSHr, a region that may be masked from the surface of native TSHr. It was produced in mouse by immunization with a recombinant GST-TSHr fusion protein, then construction of a hybridoma with the non-immunoglobulin-secreting myeloma cell line X63-Ag8-653. Amino acids 402-415 at the carboxyl terminus of the TSHr were used to construct the fusion protein.

Usage: Applications include immunoblotting (Western, ascites 1:2,000), immunohistochemistry (acetone-fixed cells, ascites 1:50), immunoprecipitation, and ELISA (1:1,000-1:1,000,000 of ascites).

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Specificity & Preparation: This antibody recognizes the extracellular domain of the human TSH receptor (TSHr). The murine monoclonal antibody A9 is specific for residues147-228 of the TSHr. This epitope is localized in the middle regions of the extracellular domain of the TSHr, an area that may be exposed at the surface of native TSHr. It was produced in mouse by immunization with a recombinant GST-TSHr fusion protein, then construction of a hybridoma with the non-immunoglobulin-secreting myeloma cell line X63-Ag8-653. Amino acids found in the middle region of the TSHr, residues 147-228, were used to construct the fusion protein.

Usage: Applications include immunoblotting (Western, ascites 1:20,000), immunohistochemistry (frozen unfixed, acetone-fixed, and periodate-lysine-paraformaldehyde-fixed sections; ascites 1:50), immunoprecipitation, and ELISA (1:1,000-1:1,000,000 of ascites).

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Specificity & Preparation: Detects (GKPIPNPLLGLDST) tag fusion protein. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.

Usage: Western Blot (Colorimetric 1:1,000-1:10,000; Chemiluminescent 1:1,000-1:30,000), ELISA (coating 1:100-1:500, primary 1:1,000-1:30,000), immunochemistry (1:100-1:400). In some cases, the antibody may be diluted further than indicated.

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