| Product Details for AB-476 |
Heparanase 1 HP130 Mouse Monoclonal (Cat. #AB-476)
Specificity & Preparation: Anti-Human heparanase 1 (HPA1) is a protein G affinity-purified monoclonal antibody raised against the 65 kDa heparanase precursor. It recognizes the C-terminal region of both the latent pro-heparanase and the active heterodimeric enzyme. In immunoblot analysis, it reacts with the 50 kDa subunit and with the 65 kDa precursor of human heparanase. The antibodies cross react with the chicken heparanase. Each vial contains 850 ?g of antibody in 500 ?l of 0.22 micron-filtered solution of 20 mM Sodium Phosphate, 150 mM NaCl, pH 7.2, containing 0.01% Thimerosal. Purity is greater than 95% on SDS-PAGE.
Usage: Applications include flow cytometry, immunohistochemistry (1:20), western blot (1:200), and immunoprecipitation.
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