DL-5-hydroxytryptophan Mouse Monoclonal, Conjugated (Cat. #AB-T077)
Specificity & Preparation: Monoclonal antibody was obtained after BALB/c mouse immunization with the conjugate DL-5-hydroxytrptophan (DL-5-HW)-Glutaraldehyde-Carriers and hybridization of spleen cells with the myeloma cell line SP2/O/Ag14. Ascites production was performed in BALB/c mice. The ascitic fluid was purified by ammonium sulfate precipitation. This antibody targets conjugated DL-5-hydroxytryptophan. This antibody does not recognize free DL-5-hydroxytryptophan.Using a conjugate DL-5-HW-Glutaraldehyde-BSA, antibody specificity was performed with an ELISA test by competition experiments with the following compounds:
Usage: Applications include immunohistochemistry / immunocytochemistry (1/1,000-1/5,000), ELISA (1/1,000-1/5,000), and immunoblotting (western blot 1/1,000-1/2,000).
Related Products: 5-Hydroxytryptophan Rabbit Polyclonal, Conjugated, Cat. #AB-T09;
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