GAD65 (B78) Human Monoclonal, biotin labeled (Cat. #BT-N12)
Specificity & Preparation: This antibody is specific for the 65-kDa isoform of glutamic acid decarboxylase (GAD65), targeting the IDDM-E2 region (amino acids 451-570), and does not bind the second isoform, GAD67. To create this cell line, peripheral blood B-cells were obtained from a donor who tested positive for GAD65 autoantibodies and were immortalized with Epstein-Barr virus. It has been conjugated to biotin via an amide bond.
Usage: Applications include immunoblotting (Western, antibody supernatant 1:100), immunoprecipitation (antibody supernatant 1:5,000-1:10,000), immunohistochemistry, and protein footprinting (10 ul serum).
Related Products: GAD65 (B78) Human Monoclonal +, Cat. #AB-N12; GAD65 (B78) Human Monoclonal, Alexa488 labeled, Cat. #FL-N12;
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