a chemical conjugate of pre-immune goat IgG antibody
and the ribosome-inactivating protein, saporin

(molecular weight 210 kDa)

This molecule is the perfect control for use with the "second" immunotoxins Mab-ZAP, Rab-ZAP, Hum-ZAP, Rat-ZAP, and Anti-M-ZAP. The secondary antibody conjugates are used with a primary antibody (a mouse monoclonal, rabbit polyclonal, human monoclonal, rat monoclonal, or IgM mouse monoclonal) to determine if the primary antibody can internalize saporin and would, therefore, be suitable for conjugation as a primary immunotoxin. Goat IgG-SAP, used in place of the secondary antibody conjugate, will give a definitive baseline for comparison of the activity of the primary antibody to internalize.

Effective Tool

• The perfect control molecule for secondary antibody conjugates
• There's no mixing of reagents; use it "as is" out of the vial
• No complicated calculations; use the same amount as the targeted toxin
• Ready to use: it comes sterile-filtered in phosphate-buffered saline
• It has the activity of non-targeted saporin, that is, almost none

Check out this article in Targeting Trends for more information. January 2002 - Control Conjugates: The Perfect Companion for Targeted Toxins, continued