Custom Conjugates are powerful tools that specifically destroy targeted cell types. This technique has important research applications in determining the function or contribution of the targeted cells in a biological system or for the removal of interfering cell types. Through the use of custom conjugates, you can eliminate any cell type.
Requirements for Successful Targeting
- A cell surface marker.
- A molecule, such as an antibody or a ligand, that binds to that marker.
Your targeting agent is conjugated to Saporin, a highly active and potent ribosome-inactivating protein.
- Select your targeting agent.
As an example, for the specific and complete removal of mature T lymphocytes, use your own anti-transferrin receptor antibody; or obtain one from the ATCC.
- Use the appropriate Secondary Conjugate to screen your targeting agent for specificity and internalization.
- Call for a quote.
Call ATS toll-free (877) 889-2288 to discuss your targeting project. All discussions and services can be covered with a confidentiality agreement.
- Prepare for stunning, powerful research results!
Examples of Uses
- Examine the role of targeted neurons in behavior, in gene expression and to determine the effect of these neurons on other neurons in the brain.
- Selective removal of subtypes of the hematopoietic system, which has well-characterized cell surface markers.
Custom conjugates are valuable tools with the potential for applications in many areas.
In vitro, custom conjugates can be used to selectively eliminate cells expressing a specific receptor/antigen. This could be very useful in various cloning and gene trapping strategies where the goal is to select for cells based on expression (or suppression of expression) of a particular gene.
In vivo, custom conjugates can be used to specifically eliminate selected types of neurons for studies of the function of those neurons and responsiveness of animals to a variety of manipulations, including:
- Behavioral studies
- Drug treatments
- Physiological studies
Screening Targeting Agents
- Use a Secondary Conjugate to test your antibody as a targeting reagent.
Also available: Custom Protein-Protein Conjugates
Check out these articles in Targeting Trends for more information: