FITC-labeled goat anti-rat IgG (H+L)can be used to verify specific binding of rat IgG to its receptor. By first incubating cells with the primary rat antibody, and then binding the FITC-labeled goat anti-rat IgG to the primary antibody, a fluorescent marker is formed that can demonstrate expression of a receptor or affinity of an antibody for its receptor. FITC is excited by 488 nm wavelength light, and emits at 525 nm.

• Rat IgG

Applications