This 1-color CFSE (carboxyfluorescein-succinimidyl ester) Compensation Kit contains negative/unstained microspheres and CFSE microspheres. Fluorescence compensation is the process wherein a portion of the primary dye signal is removed from all non-primary dye channels. Compensation is generally required whenever more than a single dye (color) is used in flow cytometric analysis. Fluorescent dyes emit light over a range of wavelengths and while the filters are optimized to collect the light from the primary dye, some light may spillover into other channels. The evaluation and setting of compensation therefore is needed to determine how much spillover occurs from one dye channel to the next and to adjust for that spillover.

• CFSE (carboxyfluorescein-succinimidyl ester) binds to amine groups on cellular proteins. Cells, once labeled with CFSE, can be stimulated in vitro and cell proliferation measured by changes in the staining intensity of the cells.

Applications