Saporin is a ribosome-inactivating protein, molecular weight 30kD, from seeds of the plant Saponaria officinalis.
Effective Tool
- Use as a control in experiments with targeted saporin immunotoxins or ligand toxins
- Saporin is safe for laboratory use under normal safety conditions
- LD50 in mice is 4 mg/kg
- Saporin does not have a method of cell entry on its own
Sample Protocol
For most targeted toxins, there are control conjugates available (see list); these are the most accurate controls to use. For a few products, saporin is used as control. Calculate amount of saporin needed on a molar basis. For example, FGF-SAP (Cat. #IT-38) has an average mol. wt. of 63 kDa, and 71% of that is saporin (approximately 1.5 molecules of saporin per molecule of cytokine). For injections of 5 µg of immunotoxin, an appropriate control quantity would be 3.6 µg.
Check out these articles in Targeting Trends for more information:
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