a chemical conjugate of affinity-purified goat anti-human
IgG
and the ribosome-inactivating protein, saporin
Hum-ZAP uses your primary human monoclonal IgG antibody to target and eliminate cells that recognize your primary antibody. This secondary conjugate uses the secondary antibody (affinity-purified goat anti-human IgG) to "piggyback" onto YOUR human primary antibody. Hum-ZAP can be utilized for screening human IgG antibodies for internalization and/or their suitability to make potent immunotoxins. When the in vitro results confirm the desired specificity, it is recommended that you order a custom conjugation of your antibody to saporin.
Fab-ZAP human (Cat. #IT-51) is also available and uses a monovalent antibody conjugate to target your human monoclonal IgG.
Elimination of Specific Cell Type
- Cells that internalize your human monoclonal antibody will be eliminated.
- Potency may vary according to the specificity and affinity of YOUR antibody to ITS receptor.
- Second immunotoxins are most effective in determining specificity of your monoclonal antibody and suitability for conjugation as a primary immunotoxin.
- ATS recommends that you order a custom conjugation of your antibody to saporin when the in vitro results confirm the desired specificity.
Protocols: Preparing for a Cytotoxicity Assay; Preparing Test Samples
Check out these articles in Targeting Trends for more information:
Please select the button below for Cat. #IT-22 Pricing in your currency. The kit includes the following controls: Goat IgG-SAP, Cat. #IT-19;
This product is available individually and as a kit (Cat. #KIT-22).
You may also be interested in these products for Hum-ZAP ^, Cat. #IT-22.
Controls: Goat IgG-SAP, Cat. #IT-19;
Related Products: Custom Conjugates; Fab-ZAP human ^, Cat. #IT-51;
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