a chemical conjugate of affinity-purified goat anti-rat IgG
and the ribosome-inactivating protein, saporin

(molecular weight 210 kDa)

Second immunotoxins are conjugations of a secondary antibody to saporin. This secondary conjugate uses the secondary antibody (affinity-purified goat anti-rat IgG) to "piggyback" onto your rat primary antibody in order to evaluate the ability of the primary antibody to internalize. Once Rat-ZAP is internalized, saporin breaks away from the targeting agent and inactivates the ribosomes, which causes protein inhibition and, ultimately, cell death.

Elimination of Specific Cell Type

• Cells that internalize your rat monoclonal antibody will be eliminated.
• Potency may vary according to the specificity and affinity of YOUR antibody to ITS receptor.
• >Second immunotoxins are most effective in determining specificity of your monoclonal antibody and suitability for conjugation as a primary immunotoxin.
• ATS recommends that you order a custom conjugation of your antibody to saporin when the in vitro results confirm the desired specificity

Protocols: Preparing for a Cytotoxicity Assay; Preparing Test Samples

Check out these articles in Targeting Trends for more information. Oct-Nov-Dec 2003 - Targeting Tools: Rat-ZAP