a chemical conjugate of affinity-purified goat anti-rat IgG
and the ribosome-inactivating protein, saporin

Secondary conjugates are conjugations of a secondary antibody to the ribosome-inactivating protein, saporin (from the seeds of the plant, Saponaria officinalis). The secondary conjugate uses the secondary antibody (affinity-purified goat anti-rat IgG) to "piggyback" onto YOUR mouse primary antibody in order to evaluate the ability of the primary antibody to internalize. Once Rat-ZAP is internalized, saporin breaks away from the targeting agent and inactivates the ribosomes, which causes protein inhibition and, ultimately, cell death.

Elimination of Specific Cell Type

• Cells that internalize your rat monoclonal antibody will be eliminated.
• Potency may vary according to the specificity and affinity of YOUR antibody to ITS receptor.
• >Second immunotoxins are most effective in determining specificity of your monoclonal antibody and suitability for conjugation as a primary immunotoxin.
• ATS recommends that you order a custom conjugation of your antibody to saporin when the in vitro results confirm the desired specificity

Protocols: Preparing for a Cytotoxicity Assay; Preparing Test Samples