Fab-ZAP rabbit

a chemical conjugate of goat anti-rabbit monovalent antibody
and the ribosome-inactivating protein, saporin

Fab-ZAP uses your primary rabbit affinity-purified polyclonal IgG antibody to target and eliminate cells that recognize your primary antibody. Fab-ZAP is made with a monovalent secondary antibody eliminating the possibility of cap formation, while preserving all the qualities that make an effective in vitro diagnostic tool. The antibodies used are affinity-purified polyclonal antibodies against both the heavy and light chain of mouse IgG. The antibody used in this product will cross-react across immunoglobulin classes and subclasses of the same species as they share the same light chain (either kappa or lambda). It also has an improved EC50 when directly compared to Rab-ZAP in a cytotoxicity assay. Fab-ZAP can be utilized for screening rabbit IgG antibodies for internalization and/or their suitability to make potent immunotoxins. When the in vitro results confirm the desired specificity, it is recommended that you order a custom conjugation of your antibody to saporin.

Rab-ZAP (Cat. #IT-05) is also available and uses a bivalent antibody conjugate to target your rabbit polyclonal IgG.

Elimination of Specific Cell Type

• Cells that internalize your rabbit IgG antibody will be eliminated.
• Potency may vary according to the specificity and affinity of YOUR antibody to ITS antigen.
• Second immunotoxins are most effective in determining specificity of your antibody and suitability for conjugation as a primary immunotoxin.
• ATS recommends that you order a custom conjugation of your antibody to saporin when the in vitro results confirm the desired specificity.

Protocols: Preparing for a Cytotoxicity Assay; Preparing Test Samples

Available Control: The recommended control for use with this product would be a non-binding primary antibody, such as an isotype control, that mimics your primary antibody targeting agent. This control antibody should be used with Fab-ZAP identically to the manner in which you test your primary antibody of interest. However, as a convenient alternative, we do offer Goat IgG-SAP (Cat #IT-19) for use as a control toxin. Goat IgG-SAP is a non-binding immunotoxin made from pre-immune, bivalent IgG attached to saporin.

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Check out these articles in Targeting Trends for more information:

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