192-Saporin (192-IgG-SAP)

192-IgG-SAP (192-Saporin)

a chemical conjugate of a mouse monoclonal antibody to rat p75^NTR
and the ribosome-inactivating protein, saporin

(molecular weight 210 kDa)

Intraventricular injection of 192-IgG-SAP (192-Saporin) results in almost complete elimination of LNGFR (p75^NTR)-positive cells in rat. 192-IgG-SAP is directed to a cell-surface antigen that is only expressed at high levels on neurons in the cholinergic basal forebrain (CBF). The antigen, p75^NTR, is not expressed on the neighboring, non-cholinergic neurons. This immunotoxin provides researchers with a powerful lesioning tool - more specific and effective than chemical, surgical or electrolytic lesioning.

To eliminate p75^NTR-expressing cells in mouse, use mu p75-SAP. To eliminate p75^NTR-expressing cells in other mammals, use ME20.4-SAP.

Elimination of Specific Cell Type

192-IgG-SAP recognizes p75^NTR-expressing cells in rat.
192-IgG-SAP specifically eliminates the following:
- Cholinergic neurons of the basal forebrain
- Medial septum
- Diagonal band of Broca
- Nucleus basalis of Meynert
- Purkinje neurons of the cerebellum

Effective Tool to Study Behavior

Permanent and selective removal of cholinergic forebrain neurons makes an important animal model for the study of the following:

Behavior (cholinergic forebrain function)
Neuronal loss (e.g. Alzheimer's disease)
Plasticity of other systems in response to loss
Replacement therapy
Drug effects and dependence

Check out these articles in Targeting Trends for more information.

Apr-May-Jun 2006 - Targeted Toxins in Pain, continued

Jul-Aug-Sep 2004 - Targeting Talk: Eliminating CBF Neurons

Apr-May-Jun 2004 - 192 IgG-Saporin-Induced Lesions Identify an Inhibitory Role in Cocaine Reward for Cholinergic Neuronal Systems in the Diagonal Band and Ventral Pallidum, continued

Jan-Feb-Mar 2004 - Cholinergic Deafferentation of the Entorhinal Cortex Impairs Working Memory for Novel, but not Familiar Stimuli in a Delayed Non-Match to Sample (DNMS) Task., continued

Jan-Feb-Mar 2004 - Targeting Talk: Not the Right Stuff

Oct-Nov-Dec 2002 - Does 192-IgG-Saporin or 5,7-DHT kill cognitive functions in the rat?, continued

Standard Pricing
IT-01-25	25 micrograms	$330
IT-01-100	100 micrograms	$1205
IT-01-250	250 micrograms	$2840
Kit Pricing includes Saporin and Control Conjugate for control
KIT-01-25	25 micrograms	$470
KIT-01-100	100 micrograms	$1700
KIT-01-250	250 micrograms	$3965

Controls: Saporin (Cat. #PR-01), Mouse IgG-SAP (Cat. #IT-18)

a chemical conjugate of a mouse monoclonal antibody to rat p75NTR and the ribosome-inactivating protein, saporin

a chemical conjugate of a mouse monoclonal antibody to rat p75^NTR
and the ribosome-inactivating protein, saporin