Anti-dopamine beta-hydroxylase-saporin (anti-DBH-SAP) is a highly specific noradrenergic lesioning agent. It specifically targets rat* cells that express dopamine beta-hydroxylase. This vesicular enzyme is exposed to the exterior milieu upon release of noradrenaline and thus allows these cells to be targeted with saporin. The specificity of the immunotoxin correlates well with uptake of the antibody when injected intraventricularly. After systemic administration, animals have a massive reduction in plasma norepinephrine levels, indicating efficient targeting and sympathectomy. Unlike other lesioning methods, this molecular lesioning agent assures definitive ablation of the target neurons.

* Also known to react with cow and sheep, other species not confirmed.

Elimination of Specific Cell Type

• Specifically targets rat cells that express dopamine beta-hydroxylase.
• All other cells left untouched.

Effective Tool to Study Behavior

Permanent and selective removal of cerebral noradrenergic innervation makes an important animal model for the study of the following:

• Drug effects (anti-hypertensives, opiates, stimulants, etc.)
• Behavior (fear, depression, food intake)
• Plasticity of other systems in response to loss
• Primary autonomic failure

Check out these articles in Targeting Trends for more information. Apr-May-Jun 2006 - Targeted Toxins in Pain, continued Apr-May-Jun 2005 - Noradrenergic inputs to the medial amygdala originate in the A1 and A2 cells groups and release norepinephrine after mating stimulation sufficient to induce pseudopregnancy, continued April 2002 - HCRT-SAP Lesion Produces Sleepiness While anti-DBH-SAP Lesion Does Not, continued, continued

Standard Pricing IT-03-25 25 micrograms $330 IT-03-100 100 micrograms $1205 IT-03-250 250 micrograms $2840 Kit Pricing includes Saporin and Control Conjugate for control KIT-03-25 25 micrograms $470 KIT-03-100 100 micrograms $1700 KIT-03-250 250 micrograms $3965

Controls: Saporin (Cat. #PR-01), Mouse IgG-SAP (Cat. #IT-18)

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ADVANCED TARGETING SYSTEMS