a chemical conjugate of peptidase-resistant [Sar9,
Met(O2)11] analog of Substance
and the ribosome-inactivating protein, saporin
targets cells expressing substance P (NK-1) receptor
SSP-SAP is a conjugation of saporin and SSP, the Sar9, Met(O2)11 analog of Substance P. These two amino acid replacements are at two sites of digestion by tissue proteases (there is a third that is still in the analog, so it does eventually get degraded). Since the targeting agent is, of course, necessary for the cytotoxic activity, SSP-SAP will diffuse farther, and thus hit more target cells. This specific analog of SP-saporin resists peptidase digestion, allowing a greater diffusion from the injection site before its metabolism. SSP-SAP eliminates cells expressing the Substance P (NK-1) receptor. Behaviors associated with pain perception are greatly affected by the injection of SSP-SAP into the spinal cord of rats. It is not suitable for retrograde transport.
SSP-SAP is now also being used to replace SP-SAP (Cat. #IT-07). Scientific advisors have given counsel for this replacement because SSP-SAP is a superior lesioning agent in many situations, due to its targeting vehicle, a protease-resistant form of substance P. An excellent example is the paper by Martin and Sloviter, J Comp Neurol 436:127-152 (2001), in which after ineffective intraparenchymal injection of SP-SAP in the hippocampus, SSP-SAP was used with tremendous efficacy. In almost all applications we expect SSP-SAP will be used at a lower dose than SP-SAP.
Limited Label License: This product is the subject of claims in a U.S. patent entitled "Substance P-Saporin (SP-SAP) Conjugates and Methods of Use Thereof" (6,063,758 and 7,741,435), owned by Advanced Targeting Systems, Inc. Purchase of this product conveys to the buyer only the non-transferable right under this patent to use the product in research conducted by the buyer.
Elimination of Specific Cell Type, In Vivo
- Substance P receptor-bearing neurons are eliminated with this reagent.
- All other cells are left untouched, even when they are the predominant cell type.
- Not suitable for retrograde transport.
To STIMULATE cells expressing the substance P receptor, use SP-CTA (Cat. #IT-39)
Effective Tool to Study Behavior
- Behaviors associated with pain perception are greatly affected by the injection of SSP-SAP into the spinal cord of rats.
Check out these articles in Targeting Trends for more information:
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This product is available individually and as a kit (Cat. #KIT-11).
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Related Products: SP-CTA ^, Cat. #IT-39;
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