a chemical conjugate of the p75^NTR monoclonal antibody (Cat. #AB-N07)
and the ribosome-inactivating protein, saporin
targets cells expressing mammalian p75^NTR

This immunotoxin provides researchers with a powerful lesioning tool — more specific and effective than chemical, surgical or electrolytic lesioning and is active in several species. Intraventricular injection of ME20.4-SAP results in elimination of low affinity nerve growth factor receptor (p75^NTR)-positive cells in rabbit. Tissue-directed injection in primates causes loss of p75^NTR-positive neurons.

Elimination of Specific Cell Type

• The antibody recognizes p75^NTR in rabbit, sheep, dog, cat, raccoon, pig and several primate species.
• ME20.4-SAP specifically eliminates cholinergic neurons of the basal forebrain and its projections to the cerebral cortex and hippocampus.

To eliminate p75^NTR-expressing cells in mouse, use mu p75-SAP. To eliminate p75^NTR-expressing cells in rat, use 192-IgG-SAP.

Effective Tool to Study Behavior

Permanent and selective removal of cholinergic forebrain neurons makes an important animal model for the study of the following:

• Behavior (cholinergic forebrain function)
• Neuronal loss (e.g. Alzheimer's disease)
• Plasticity of other systems in response to loss
• Replacement therapy
• Drug effects and dependence