mu p75-SAP

mu p75-SAP

a chemical conjugate of the affinity-purified rabbit polyclonal antibody p75^NTR (Cat. #AB-N01AP)
and the ribosome-inactivating protein, saporin

(molecular weight 210 kDa)

Conjugation of a p75^NTR antibody to saporin has produced a cytotoxin that eliminates the CBF neurons, while sparing neighboring neurons that express GAD, calbindin and parvalbumin. This molecule eliminates cholinergic neurons in the basal forebrain of mice in the same manner that 192-IgG-SAP does in the rat.

Elimination of Specific Cell Type

Mouse p75^NTR-positive cells

To eliminate p75^NTR-expressing cells in rat, use 192-IgG-SAP. To eliminate p75^NTR-expressing cells in other mammals, use ME20.4-SAP.

Effective Tool to Study Behavior

Permanent and selective removal of cholinergic forebrain neurons makes an important animal model and can be used in conjunction with knockout models for the study of the following:

Behavior (cholinergic forebrain function)
Neuronal loss (e.g. Alzheimer’s disease)
Plasticity of other systems in response to loss
Replacement therapy
Drug effects and dependence

April 5, 2004

Thank you for your interest in our immunotoxin, mu p75-SAP, (Cat. #IT-16). As you may know, this product has been on backorder for the past five months. The delay in producing mu p75-SAP was caused by the declining specificity of the antibody we were using as the targeting agent, a rat monoclonal to the mouse NGFr. After several failed attempts to regenerate this monoclonal, we decided to use a different antibody for the targeting agent. The new and more active version of mu p75-SAP utilizes an affinity-purified rabbit polyclonal to the mouse NGFr (Cat. #AB-N01AP). As you will see in the data sheet for the new version of this immunotoxin, the ED50 for the new product is in the picomolar range compared to an ED50 in the nanomolar range for the previous product (see “Previous Data Sheet”).

The new kit includes equal aliquots of saporin and the affinity-purified rabbit polyclonal antibody. The kit and antibody will be available on April 20; the immunotoxin alone is available immediately.

Check out these articles in Targeting Trends for more information.

Apr-May-Jun 2008 - Selective lesion of basal forebrain cholinergic neurons in mice with the mu p75-saporin immunotoxin: Neuroanatomy and behavior, continued

Jan-Feb-Mar 2007 - Cholinergic Immunolesioning Produced Tangle-like Inclusions in TgCRND8 Brain, continued

Apr-May-Jun 2004 - Targeting Tools: mu p75-SAP improved

October 2000 - Targeting Tools: Mouse-Specific p75 Immunotoxins

Standard Pricing
IT-16-50	50 micrograms	$385
IT-16-100	100 micrograms	$700
IT-16-250	250 micrograms	$1655
Kit Pricing includes Saporin, Antibody, and Control Conjugate for control
KIT-16-50	50 micrograms	$880
KIT-16-100	100 micrograms	$1640
KIT-16-250	250 micrograms	$3820

Controls: Saporin (Cat. #PR-01), affinity-purified Anti-NGFr (Cat. #AB-N01AP), Rabbit IgG-SAP (Cat. #IT-35)

a chemical conjugate of the affinity-purified rabbit polyclonal antibody p75NTR (Cat. #AB-N01AP) and the ribosome-inactivating protein, saporin

a chemical conjugate of the affinity-purified rabbit polyclonal antibody p75^NTR (Cat. #AB-N01AP)
and the ribosome-inactivating protein, saporin