Does 192-IgG-Saporin or 5,7-DHT kill cognitive functions in the rat?
(continued on page 6)

Contributed by Jean-Christophe Cassel, PhD, Laboratoire de Neurosciences Cognitives et Comportementales, UMR 7521 Université Louis Pasteur/CNRS, Strasbourg, France & Institut für Experimentelle und Klinische Pharmakologie und Toxikologie der Universität Freiburg, Neuropharmakologisches Labor, Freiburg, Germany. Dr. Cassel and colleagues are the authors of four publications released this quarter using 192-Saporin.

Product information related to cover article: 192-IgG-SAP (Cat. #IT-01)

SP-SAP Efficacy Reported at IASP 10th World Congress on Pain

Targeting Talk: Custom Conjugations

Part I: Immunotoxins (Antibody-Saporin conjugates)

• How do I know my antibody will work in an immunotoxin?
• How much antibody do I need to provide for a custom conjugation?
• How much of the saporin conjugate will that give me?
• What is the ratio of saporin to antibody?
• What chemistry and conjugation conditions are involved in the preparation of a custom immunotoxin?
• What quality control is involved? Will you provide product specifications such as average saporin to antibody ratio?
• What is the cost of a custom immunotoxin preparation? How long will it take to complete?

• Easton A, Ridley RM, Baker HF, Gaffan D (2002) Unilateral lesions of the cholinergic Basal forebrain and fornix in one hemisphere and inferior temporal cortex in the opposite hemisphere produce severe learning impairments in rhesus monkeys. Cereb Cortex 12(7):729-736. (ME20.4-SAP, Cat. #IT-15)
• Johnson DA, Zambon NJ, Gibbs RB (2002) Selective lesion of cholinergic neurons in the medial septum by 192 IgG-saporin impairs learning in a delayed matching to position T-maze paradigm. Brain Res 943(1):132-141. (192-IgG-SAP, Cat. #IT-01)
• Berchtold NC, Kesslak JP, Cotman CW (2002) Hippocampal brain-derived neurotrophic factor gene regulation by exercise and the medial septum. J Neurosci Res 68(5):511-521. (192-IgG-SAP, Cat. #IT-01 or OX7-SAP, Cat. #IT-02)
• Cassel J, Gaurivaud M, Lazarus C, Bertrand F, Galani R, Jeltsch H (2002) Grafts of fetal septal cells after cholinergic immunotoxic denervation of the hippocampus: a functional dissociation between dorsal and ventral implantation sites. Neuroscience 113(4):871-882. (192-IgG-SAP, Cat. #IT-01)
• Porreca F, Burgess SE, Gardell LR, Vanderah TW, Malan TP Jr, Ossipov MH, Lappi DA, Lai J (2001) Inhibition of neuropathic pain by selective ablation of brainstem medullary cells expressing the µ-opioid receptor. J Neurosci 21(14):5281-5288. (Dermorphin-SAP, Cat. #IT-12)
• Truitt WA, Coolen LM (2002) Identification of a potential ejaculation generator in the spinal cord. Science 297(5586):1566-1569. (SSP-SAP, Cat. #IT-11)

Recent Scientific References

• Fraley GS, Dinh TT, Ritter S (2002) Immunotoxic catecholamine lesions attenuate 2DG-induced increase of AGRP mRNA. Peptides 23(6):1093-1099. (Anti-DBH-SAP, Cat. #IT-03)
• Zhu XO, de Permentier PJ, Waite PM (2002) Cholinergic depletion by IgG 192-saporin retards development of rat barrel cortex. Brain Res Dev Brain Res 136:1-16. (192-IgG-SAP, Cat. #IT-01)
• Simons CT, Gogineni AG, Iodi Carstens M, Carstens E (2002) Reduced aversion to oral capsaicin following neurotoxic destruction of superficial medullary neurons expressing NK-1 receptors. Brain Res 945:139-143. (SP-SAP, Cat. #IT-07)
• Li X, Conklin D, Ma W, Zhu X, Eisenach JC (2002) Spinal noradrenergic activation mediates allodynia reduction from an allosteric adenosine modulator in a rat model of neuropathic pain. Pain 97:117-125. (Anti-DBH-SAP, Cat. #IT-03)
• Galani R, Jeltsch H, Lehmann O, Bertrand F, Cassel JC (2002) Effects of 192 IgG-saporin on acetylcholinesterase histochemistry in male and female rats. Brain Res Bull 58(2):179-186. (192-IgG-SAP, Cat. #IT-01)
• Galani R, Lehmann O, Bolmont T, Aloy E, Bertrand F, Lazarus C, Jeltsch H, Cassel JC (2002) Selective immunolesions of CH4 cholinergic neurons do not disrupt spatial memory in rats. Physiol Behav 76:75-90. (192-IgG-SAP, Cat. #IT-01)
• Lehmann O, Jeltsch H, Lazarus C, Tritschler L, Bertrand F, Cassel JC (2002) Combined 192 IgG-saporin and 5,7-dihydroxytryptamine lesions in the male rat brain: A neurochemical and behavioral study. Pharmacol Biochem Behav 72(4):899-912. (192-IgG-SAP, Cat. #IT-01)
• Lehmann O, Bertrand F, Jeltsch H, Morer M, Lazarus C, Will B, Cassel JC (2002) 5,7-DHT-induced hippocampal 5-HT depletion attenuates behavioural deficits produced by 192 IgG-saporin lesions of septal cholinergic neurons in the rat. Eur J Neurosci 15(12):1991-2006. (192-IgG-SAP, Cat. #IT-01)

Abstracts from Forum of European Neuroscience (FENS): July 13-17, Paris France

Abstracts from IASP 10th World Congress on Pain: August 17-22, San Diego California

Does 192-IgG-Saporin or 5,7-DHT kill cognitive functions in the rat?
(continued from page 1)

Targeting Teaser Winners from last issue

• anti-SERT-SAP (Cat. #IT-23)

Targeting Teaser (Jumble)