Biotinylated targeting: A viable option?
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For the IB4-SAP illustration (Figure 1), our thanks to Christopher N. Honda, Ph.D., Associate Professor, Department of Neuroscience, University of Minnesota, 6-145 Jackson Hall, 321 Church Street, Minneapolis, MN 55455

Product information related to cover article: IB4-SAP (Cat. #IT-10), Biotinylation Service

Funding Awarded for SBIR Phase II Grant

Malia and Sam Kohls: Has it been a YEAR already?

Targeting Tickler

• Gerashchenko K, Blanco-Centurion C, Greco MA, Shiromani PJ (2003) Effects of lateral hypothalamic lesion with the neurotoxin hypocretin-2-saporin on sleep in Long-Evans rats. Neuroscience 116:223-235. (Orexin-SAP, Cat. #IT-20)
• Riley J, Lin LH, Chianca DA, Talman WT (2002) Ablation of NK1 receptors in rat nucleus tractus solitarii blocks baroreflexes. Hypertension 40(6):823-826. (SP-SAP, Cat. #IT-07)
• Silveira DC, Cha BH, Holmes GL (2002) Effects of lesions of basal forebrain cholinergic neurons in newborn rats on susceptibility to seizures. Dev Brain Res 139:277-283. (192-IgG-SAP, Cat. #IT-01)
• Fraley GS, Ritter S (2003) Immunolesion of norepinephrine and epinephrine afferents to medial hypothalamus alters basal and 2-deoxy-D-glucose-induced neuropeptide Y and agouti gene-related protein messenger ribonucleic acid expression in the arcuate nucleus. Endocrinology 144(1):75-83. (Anti-DBH-SAP, Cat. #IT-03)
• Berntson GG, Shafi R, Sarter M (2002) Specific contributions of the basal forebrain corticopetal cholinergic system to electroencephalographic activity and sleep/waking behaviour. Eur J Neurosci 16(12):2453-2461. (192-IgG-SAP, Cat. #IT-01)

Request your 2003 Catalog online.

Recent Scientific References

• Birthelmer A, Ehret A, Amtage F, Förster S, Lehmann O, Jeltsch H, Cassel JC, Jackisch R (2003) Neurotransmitter release and its presynaptic modulation in the rat hippocampus after selective damage to cholinergic or/and serotonergic afferents. Brain Res Bull 59(5):371-381. (192-IgG-SAP, Cat. #IT-01)
• Stornetta RL, Rosin DL, Wang H, Sevigny CP, Weston MC, Guyenet PG (2003) A group of glutaminergic interneurons expressing high levels of both neurokinin-1 receptors and somatostatin identifies the region of the pre-Bötzinger complex. J Comp Neurol 455(4):499-512. (SSP-SAP, Cat. #IT-11)
• Lamprea MR, Cardenas FP, Silveira R, Walsh TJ, Morato S (2003) Effects of septal cholinergic lesion on rat exploratory behavior in an open-field. Braz J Med Biol Res 36(2):233-238. (192-IgG-SAP, Cat. #IT-01)
• Salo PT, Hogervorst T, Seerattan RA, Rucker D, Bray RC (2002) Selective joint denervation promotes knee osteoarthritis in the aging rat. J Orthop Res 20(6):1256-1264. (OX7-SAP, Cat. #IT-02)
• Mantyh PW (2002) Neurobiology of substance P and the NK1 receptor. J Clin Psychiatry 63(Suppl 11):6-10. (SP-SAP, Cat. #IT-07)

Targeting Talk: Retrograde Transport

• I'm interested in using SAP to eliminate cells through retrograde transport, like OX7-SAP and IB4-SAP have been used. Can you explain how retrograde transport works and if it is possible for this to work with dermorphin-SAP? What determines whether a targeted toxin will be able to be used in retrograde transport?
• If a targeted toxin cannot be used in retrograde transport, will it only kill cell bodies in the injection site or will it also kill terminals?
  

Biotinylated materials
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Targeting Teaser Winners from last issue

• Avidinylated-SAP (Cat. #IT-09)
• Biotinylation Service

Targeting Teaser (Jumble)