192 IgG-Saporin-Induced Lesions Identify an Inhibitory Role in Cocaine Reward for Cholinergic Neuronal Systems in the Diagonal Band and Ventral Pallidum
(continued on page 6)

Contributed by Dr. James Smith, Wake Forest University School of Medicine, Department of Physiology and Pharmacology, Medical Center Blvd, Winston-Salem NC 27157

Product information related to cover article: 192-IgG-SAP (Cat. #IT-01)

Ten Years and Growing Strong

Accomplished Scientist. . . Talented Artist - Rosalind Ridley

Doors of San Marco basilica (watercolor)

• Gerashchenko D, Murillo-Rodriguez E, Lin L, Xu M, Hallett L, Nishino S, Mignot E, Shiromani PJ (2003) Relationship between CSF hypocretin levels and hypocretin neuronal loss. Exp Neurol 184(2):1010-1016. (Orexin-SAP, Cat. #It-20)
• Chudasama Y, Dalley JW, Nathwani F, Bouger P, Robbins TW (2004) Cholinergic modulation of visual attention and working memory: Dissociable effects of basal forebrain 192-IgG-saporin lesions and intraprefrontal infusions of scopolamine. Learn Mem 11(1):78-86. (192-IgG-SAP, Cat. #IT-01)
• Winters BD, Robbins TW, Everitt BJ (2004) Selective cholinergic denervation of the cingulate cortex impairs the acquisition and performance of a conditional visual discrimination in rats. Eur J Neurosci 19(2):490-496. (192-IgG-SAP, Cat. #IT-01)
• Nattie EE, Li A, Richerson GB, Lappi DA (2004) Medullary serotonergic neurons and adjacent neurons that express neurokinin-1 receptors are both involved in chemoreception in vivo. J Physiol 556(1):235-253. (SP-SAP, Cat. #IT-07 and Anti-SERT-SAP, Cat. #IT-23)
• Fargo KN, Sengelaub DR (2004) Testosterone manipulation protects motoneurons from dendritic atrophy after contralateral motoneuron depletion. J Comp Neurol 469(1):96-106. (CTB-SAP, Cat. #IT-14)
• Zhang Y, Berger SA (2004) Increased calcium influx and ribosomal content correlate with resistance to endoplasmic reticulum stress-induced cell death in mutant leukemia cell lines. J Biol Chem 279(8):6507-6516. (Saporin, Cat. #PR-01)

Recent Scientific References

• Buffo A, Carulli D, Rossi F, Strata P (2003) Extrinsic regulation of injury/growth-related gene expression in the inferior olive of the adult rat. Eur J Neurosci 18(8):2146-2158. (192-IgG-SAP, Cat. #IT-01)
• Janisiewicz AM, Baxter MG (2003) Transfer effects and conditional learning in rats with selective lesions of medial septal/diagonal band cholinergic neurons. Behav Neurosci 117(6):1342-1352. (192-IgG-SAP, Cat. #IT-01)
• Janisiewicz AM, Jackson O, Firoz EF, Baxter MG (2004) Environment-spatial conditional learning in rats with selective lesions of medial septal cholinergic neurons. Hippocampus 14:265-273. (192-IgG-SAP, Cat. #IT-01)
• Parent MB, Baxter MG (2004) Septohippocampal acetylcholine: involved in but not necessary for learning and memory? Learn Mem 11:9-20. (192-IgG-SAP, Cat. #IT-01)
• Vuckovich JA, Semel ME, Baxter MG (2004) Extensive lesions of cholinergic basal forebrain neurons do not impair spatial working memory. Learn Mem 11:87-94. (192-IgG-SAP, Cat. #IT-01)
• Frick KM, Jeansok JK, Baxter MG (2004) Effects of complete immunotoxin lesions of the cholinergic basal forebrain on fear conditioning and spatial learning. Hippocampus 14:244-254. (192-IgG-SAP, Cat. #IT-01)

Targeting Talk: Streptavidin versus Avidin

• I recently tried to order avidinylated-SAP and was told that this product has been replaced with a new product, streptavidin-SAP (streptavidin-ZAP). Why did you replace avidinylated-SAP?
• I use avidinylated-SAP to demonstrate that my antibody internalizes. It worked quite well for me.

192 IgG-Saporin-Induced Lesions Identify an Inhibitory Role in Cocaine Reward
(continued from page 1)

Targeting Tools: Featured Products

• mu p75-SAP, improved (Cat. #IT-16)
• Anti-Tac-SAP (Cat. #IT-24 and Cat. #IT-29)

Targeting Teaser (Jumble)