The Discovery of Saporin (continued on page 6)

Contributed by Dr. Fiorenzo Stirpe, Dipartimento di Patologia sperimentale, Università di Bologna, I-40125 Bologna, Italy

Product information related to cover article: Saporin (Cat. #PR-01)

SAP Facts

Drug Development Update (article)

Accomplished Scientist. . . Talented Artist - Rosalind Ridley

Doorway of Campo Sant’Angelo

• Erhardt C, Galani R, Jeltsch H, Cassel JC, Klosen P, Menet JS, Pevet P, Challet E (2004) Modulation of photic resetting in rats by lesions of projections to the suprachiasmatic nuclei expressing p75 neurotrophin receptor. Eur J Neurosci 19(7):1773-1788. (192-IgG-SAP, Cat. #IT-01)
• Dalley JW, Theobald DE, Bouger P, Chudasama Y, Cardinal RN, Robbins TW (2004) Cortical cholinergic function and deficits in visual attentional performance in rats following 192 IgG-Saporin-induced lesions of the medial prefrontal cortex. Cereb Cortex 14(8):922-932. (192-IgG-SAP, Cat. #IT-01)
• Smith JE, Co C, Yin X, Sizemore GM, Liguori A, Johnson WE 3rd, Martin TJ (2004) Involvement of cholinergic neuronal systems in intravenous cocaine self-administration. Neurosci Biobehav Rev 27(8):841-850. (192-IgG-SAP, Cat. #IT-01)
• Rajakumar N, Leung LS, Ma J, Rajakumar B, Rushlow W (2004) Altered neurotrophin receptor function in the developing prefrontal cortex leads to adult-onset dopaminergic hyperresponsivity and impaired prepulse inhibition of acoustic startle. Biol Psychiatry 55(8):797-803. (192-IgG-SAP, Cat. #IT-01)
• Yezierski RP, Yu CG, Mantyh PW, Vierck CJ, Lappi DA (2004) Spinal neurons involved in the generation of at-level pain following spinal injury in the rat. Neurosci Lett 361(1-3):232-236.(SSP-SAP, Cat. #IT-11)
• Blanco-Centurion C, Gerashchenko D, Salin-Pascual RJ, Shiromani PJ (2004) Effects of hypocretin2-saporin and antidopamine-beta-hydroxylase-saporin neurotoxic lesions of the dorsolateral pons on sleep and muscle tone. Eur J Neurosci 19(10):2741-2752. (192-IgG-SAP, Cat. #IT-01; Anti-DBH-SAP, Cat. #IT-03; Orexin-SAP, Cat #IT-20)

Recent Scientific References

• Li AJ, Ritter S (2004) Glucoprivation increases expression of neuropeptide Y mRNA in hindbrain neurons that innervate the hypothalamus. Eur J Neurosci 19(8):2147-2154. (Anti-DBH-SAP, Cat. #IT-03)
• Chang Q, Gold PE (2004) Impaired and spared cholinergic functions in the hippocampus after lesions of the medial septum/vertical limb of the diagonal band with 192 IgG-saporin. Hippocampus 14(2):170-179. (192-IgG-SAP, Cat. #IT-01)
• Beninger RJ, Dringenberg HC, Boegman RJ, Jhamandas K (2001) Cognitive effects of neurotoxic lesions of the nucleus basalis magnocellularis in rats: differential roles for corticopetal versus amygdalopetal projections. Neurotox Res 3(1):7-21. (192-IgG-SAP, Cat. #IT-01)
• Nishiguchi J, Sasaki K, Seki S, Chancellor MB, Erickson KA, de Groat WC, Kumon H, Yoshimura N (2004) Effects of isolectin B4-conjugated saporin, a targeting cytotoxin, on bladder overactivity induced by bladder irritation. Eur J Neurosci 20(2):474-482. (IB4-SAP, Cat. #IT-10)
• Lappi DA, Wiley RG (2004) Immunotoxins and neuropeptide-toxin conjugates experimental applications. Mini Rev Med Chem 4(5):585-595.
• Dudkin KN, Chueva IV, Makarov FN (2003) [Interaction between sensory and cognitive processes in visual recognition: the role of the associative areas of the cerebral cortex] Russian Ross Fiziol Zh Im I M Sechenova 89(10):1226-1239. (ME20.4-SAP, Cat. #IT-15)

Targeting Talk: Eliminating CBF Neurons

• What dosage of 192-Saporin should be used in the lateral ventricle to eliminate cholinergic neurons in the basal forebrain, including substantia innominata (SI)? I read that Calza et al (1) used 2 or 3 micrograms/4.5 ul and found this was highly effective.
• Should we expect to be able to kill all or almost all ChAT SI neurons?
• Is there another toxin that will eliminate the remaining ChAT SI neurons?

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The Discovery of Saporin (continued from page 1)

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