Noradrenergic inputs to the medial amygdala originate in the A1 and A2 cells groups and release norepinephrine after mating stimulation sufficient to induce pseudopregnancy
(continued on page 6)

Contributed by Lesley E. Northrop, Nicole Cameron and Mary Erskine,
Department of Biology, Boston University.

Product information related to cover article: Anti-DBH-SAP (Cat. #IT-03)

SP-SAP : How It’s Different From Substance P Antagonists (article)

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Recent Scientific References

• Nolan BC, Freeman JH Jr (2005) Purkinje cell loss by OX7-saporin impairs excitatory and inhibitory eyeblink conditioning. Behav Neurosci 119(1):190-201. (OX7-SAP, Cat. #IT-02)
• Mohapel P, Leanza G, Kokaia M, Lindvall O (2005) Forebrain acetylcholine regulates adult hippocampal neurogenesis and learning. Neurobiol Aging 26:939-946. (192-IgG-SAP, Cat. #IT-01)
• Seki S, Erickson KA, Seki M, Nishizawa O, Igawa Y, Ogawa T, de Groat WC, Chancellor MB, Yoshimura N (2005) Elimination of rat spinal neurons expressing neurokinin 1 receptors reduces bladder overactivity and spinal c-fos expression induced by bladder irritation. Am J Physiol Renal Physiol 288(3):F466-F473. (SSP-SAP, Cat. #IT-11)
• Harrell LE, Parsons DS, Kolasa K (2005) The effect of central cholinergic and noradrenergic denervation on hippocampal sympathetic ingrowth and apoptosis-like reactivity in the rat. Brain Res 1033(1):68-77. (192-IgG-SAP, Cat. #IT-01)
• Yoder RM, Pang KC (2005) Involvement of GABAergic and cholinergic medial septal neurons in hippocampal theta rhythm. Hippocampus 15(3):381-392. (192-IgG-SAP, Cat. #IT-01)
• Marques Pereira P, Cosquer B, Schimchowitsch S, Cassel JC (2005) Hebb-Williams performance and scopolamine challenge in rats with partial immunotoxic hippocampal cholinergic deafferentation. Brain Res Bull 64(5):381-394. (192-IgG-SAP, Cat. #IT-01)

Recent Scientific References

• Bugarith K, Dinh TT, Li AJ, Speth RC, Ritter S (2005) Basomedial hypothalamic injections of neuropeptide Y conjugated to saporin selectively disrupt hypothalamic controls of food intake. Endocrinology 146(3):1179-1191. (NPY-SAP, Cat. #IT-28)
• Jonasson Z, Cahill JF, Tobey RE, Baxter MG (2004) Sexually dimorphic effects of hippocampal cholinergic deafferentation in rats. Eur J Neurosci 20(11):3041-3053. (192-IgG-SAP, Cat. #IT-01)
• Gerashchenko D, Chou TC, Blanco-Centurion CA, Saper CB, Shiromani PJ (2004) Effects of lesions of the histaminergic tuberomammillary nucleus on spontaneous sleep in rats. Sleep 27(7):1275-1281. (Orexin-SAP, Cat. #IT-20)
• Chiken S, Tokuno H (2005) Impairment of skilled forelimb use after ablation of striatal interneurons expressing substance P receptors in rats: an analysis using a pasta matrix reaching task. Exp Brain Res 162(4):532-536. (SP-SAP, Cat. #IT-07)
• Kim I, Wilson RE, Wellman CL (2005) Aging and cholinergic deafferentation alter GluR1 expression in rat frontal cortex. Neurobiol Aging 26(7):1073-1081. (192-IgG-SAP, Cat. #IT-01)

Targeting Talk: Cytotoxicity of Unbound Saporin

• We are re-examining some data collected using an immunotoxin not prepared by your company (VChAT-sap). Our results in vivo indicated that there were nonspecific effects although the creators claimed it was specific. We ran a Western Blot and determined that about half the saporin was not bound to the antibody. This may have been the problem but I want to confirm the cytotoxicity of unbound saporin. Can you confirm that? Further, the antibody in question never bound selectively in ferret tissue. Does this suggest a problem as well? Can you give me information on the best way to design and test an immunotoxin.

Norepinephrine Release After Mating Stimulation (continued from page 1)

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