Effects of Intraseptal Orexin-Saporin on Spatial Memory (continued on page 6)

by Kevin Pang and Heidi Smith, Bowling Green State University, Dept Psychology, Bowling Green, Ohio

Product information related to cover article: Orexin-SAP (Cat. #IT-20)

17th Annual Spring Brain Conference (www.springbrain.org)

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Recent Scientific References

• Abe T, Ohshita N, Sugiyo S, Moritani M, Kobayashi M, Takemura M (2005) Elimination of neurokinin-1 receptor neurons in caudal nucleus reverses the effects of systemic bicuculline on c-Fos expression in rat trigeminal sensory nucleus: I. High intensity electrical stimulation of the trigeminal ganglion. Neuroscience 133(3):739-747. (Cat. #IT-07)
• Castle M, Comoli E, Loewy AD (2005) Autonomic brainstem nuclei are linked to the hippocampus. Neuroscience 134(2):657-669. (Cat. #IT-01; Cat. #IT-03)
• Chance WT, Dayal R, Friend LA, Sheriff S (2005) Possible role of CRF peptides in burn-induced hypermetabolism. Life Sci [Aug 23 Epub]. (Cat. #IT-13)
• Hartonian I, de Lacalle S (2005) Compensatory changes in cortical cholinergic innervation in the rat following an immunotoxic lesion. Restor Neurol Neurosci 23(2):87-96. (Cat. #IT-01)
• Kamke MR, Brown M, Irvine DR (2005) Origin and immunolesioning of cholinergic basal forebrain innervation of cat primary auditory cortex. Hear Res 206(1-2):89-106. (Cat. #IT-15)
• Kuczewski N, Aztiria E, Leanza G, Domenici L (2005) Selective cholinergic immunolesioning affects synaptic plasticity in developing visual cortex. Eur J Neurosci 21(7):1807-1814. (Cat. #IT-01)
• Thinschmidt JS, Frazier CJ, King MA, Meyer EM, Papke RL (2005) Septal innervation regulates the function of alpha7 nicotinic receptors in CA1 hippocampal interneurons. Exp Neurol 195(2):342-352. (Cat. #IT-01)

Recent Scientific References

• Koh S, Santos TC, Cole AJ (2005) Susceptibility to seizure-induced injury and acquired microencephaly following intraventricular injection of saporin-conjugated 192 IgG in developing rat brain. Exp Neurol 194(2):457-466. (Cat. #IT-01)
• Ridley RM, Baker HF, Leow-Dyke A, Cummings RM (2005) Further analysis of the effects of immunotoxic lesions of the basal nucleus of Meynert reveals substantial impairment on visual discrimination learning in monkeys. Brain Res Bull 65(5):433-442. (Cat. #IT-15)
• Ross RS, McGaughy J, Eichenbaum H (2005) Acetylcholine in the orbitofrontal cortex is necessary for the acquisition of a socially transmitted food preference. Learn Mem 12(3):302-306. (Cat. #IT-01)
• Wu M, Kc P, Mack SO, Haxhiu MA (2005) Ablation of vagal preganglionic neurons innervating the extra-thoracic trachea affects ventilatory responses to hypercapnia and hypoxia. Respir Physiol Neurobiol [Aug 10 Epub]. (Cat. #IT-14)
• Zhang RX, Wang L, Liu B, Qiao JT, Ren K, Berman BM, Lao L (2005) Mu opioid receptor-containing neurons mediate electroacupuncture-produced anti-hyperalgesia in rats with hind paw inflammation. Brain Res 1048(1-2):235-240. (Cat. #IT-12)
• Suzuki R, Rahman W, Rygh LJ, Webber M, Hunt SP, Dickenson AH (2005) Spinal-supraspinal serotonergic circuits regulating neuropathic pain and its treatment with gabapentin. Pain 117(3):292-303. (Cat. #IT-07)

Targeting Talk: Dose Ranging

• We just completed surgeries where we implanted third ventricular cannulas and temporary bilatera cannulas directed into the nucleus tractus solitarius in the brainstem of animals. We injected either the Blank-SAP control toxin or the experimental material Oxytocin-SAP into the bilateral NTS cannulae over a 30-second period. However, within the next week-two weeks post-surgery, we lost 13 of the 19 animals treated; they appeared not to be able to groom properly and lost over 20% of their body weight. This was apparent in both the Blank-SAP and the Oxytocin-SAP groups. We gave a dose of 40 ng/300 nl for each of the reagents. This dose was determined based on a published article using another of ATS’s targeted toxins. I’m very surprised by my results. Can you offer any explanation/advice?
• Is there some kind of formula that one can use that will help determine a starting point for establishing a range of doses to test in animals prior to initiating a study? For example, if the targeted toxin is administered intravenously, does it take more or less material than when administered directly into tissue?

This brilliant artistic expression is a creation of this issue’s Nobel Prize winner as featured in the Targeting Teaser on the back cover. The title of this piece is Hamadryad.

Effects of Intraseptal Orexin-SAP on Spatial Memory (continued from page 1)

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