Basomedial Hypothalamic Injections of Neuropeptide Y Conjugated to Saporin Selectively Disrupt Hypothalamic Controls of Food Intake
(continued on page 6)

This article is a summary of data presented in the reference below. Figures 1-4 are taken from that article. This work was funded by NS045520 and DK40498 to S. Ritter.
Bugarith K, Dinh TT, Li AJ, Speth RC, Ritter S. (2005) Endocrinology 146:1179-1191.

Product information related to cover article: NPY-SAP (Cat. #IT-28)

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Recent Scientific References

• Collins BE, Blixt O, Han S, Duong B, Li H, Nathan JK, Bovin N, Paulson JC (2006) High-affinity ligand probes of CD22 overcome the threshold set by cis ligands to allow for binding, endocytosis, and killing of B cells. J Immunol 177(5):2994-3003. (Cat. #IT-27)
• Kanai T, Uraushihara K, Totsuka T, Nemoto Y, Fujii R, Kawamura T, Makita S, Sawada D, Yagita H, Okumura K, Watanabe M (2006) Ameliorating effect of saporin-conjugated anti-CD11b monoclonal antibody in a murine T-cell-mediated chronic colitis. J Gastroenterol Hepatol 21(7):1136-1142. (Cat. #IT-06)
• Fraley GS (2006) Immunolesions of glucoresponsive projections to the arcuate nucleus alter glucoprivic-induced alterations in food intake, luteinizing hormone secretion, and GALP mRNA, but not sex behavior in adult male rats. Neuroendocrinology 83(2):97-105. (Cat. #IT-03)
• Adam PJ, Terrett JA, Steers G, Stockwin L, Loader JA, Fletcher GC, Lu LS, Leach BI, Mason S, Stamps AC, Boyd RS, Pezzella F, Gatter KC, Harris AL (2006) CD70 (TNFSF7) is expressed at high prevalence in renal cell carcinomas and is rapidly internalised on antibody binding. Br J Cancer 95(3):298-306. (Cat. #IT-22)
• Parikh V, Sarter M (2006) Cortical choline transporter function measured in vivo using choline-sensitive microelectrodes: clearance of endogenous and exogenous choline and effects of removal of cholinergic terminals. J Neurochem 97(2):488-503. (Cat. #IT-01; Cat. #IT-18)
• Ritter S, Dinh TT, Li AJ (2006) Hindbrain catecholamine neurons control multiple glucoregulatory responses. Physiol Behav [Epub Jul 31]. (Cat. #IT-03)
• Grimaldi P, Rossi F (2006) Lack of neurogenesis in the adult rat cerebellum after Purkinje cell degeneration and growth factor infusion. Eur J Neurosci 23(10):2657-2668. (Cat. #IT-01)

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• Blanco-Centurion C, Xu M, Murillo-Rodriguez E, Gerashchenko D, Shiromani AM, Salin-Pascual RJ, Hof PR,Shiromani PJ (2006) Adenosine and sleep homeostasis in the Basal forebrain. J Neurosci 26(31):8092-8100. (Cat. #IT-01)
• Fransson J, Borrebaeck CA (2006) The nuclear DNA repair protein Ku70/80 is a tumor-associated antigen displaying rapid receptor mediated endocytosis. Int J Cancer [Epub Aug 23]. (Cat. #IT-04)
• Nattie E, Li A (2006) Neurokinin-1 receptor expressing neurons in the ventral medulla are essential for normal central and peripheral chemoreception in the conscious rat. J Appl Physiol [Epub Aug 10]. (Cat. #IT-11; Cat. #IT-18)
• Nolan BC, Freeman JH (2006) Purkinje cell loss by OX7-saporin impairs acquisition and extinction of eyeblink conditioning. Learn Mem 13(3):359-365. (Cat. #IT-02)
• Rygh LJ, Suzuki R, Rahman W, Wong Y, Vonsy JL, Sandhu H, Webber M, Hunt S, Dickenson AH (2006) Local and descending circuits regulate long-term potentiation and zif268 expression in spinal neurons. Eur J Neurosci 24(3):761-772. (Cat. #IT-07; Cat. #PR-01)
• Vera-Portocarrero LP, Zhang ET, Ossipov MH, Xie JY, King T, Lai J, Porreca F (2006) Descending facilitation from the rostral ventromedial medulla maintains nerve injury-induced central sensitization. Neuroscience 140(4):1311-1320. (Cat. #IT-12)

Targeting Talk: Anti-DBH-SAP Administration

• We injected anti-DBH-SAP into the hypothalamus of Sprague-Dawley rats and sacrificed them 2 weeks later. We did not see any reduction in the DBH fiber staining.
  When the drug arrived, we aliquoted it in 1-µl snap-cap tubes on ice, and stored them at -80°C. For injections, a 1-µl aliquot was diluted to alittle over 10 µl so that we had a final concentration of 1 µg/10 µl.We administered two injections of 100 nl on each side (with 10 ng of anti-DBH-SAP) using a 0.5-µl Hamilton syringe attached to a stereotax. The needle was a 33-gauge with a blunt tip. I tried previously to use glass micropipette tips attached to a Hamilton syringe with the line filled with mineral oil, but found that the actual volume displacement was too unreliable.

NPY- SAP Selectively Disrupts Hypothalamic Controls of Food Intake
(continued from page 1)

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