Targeted Therapy for Chronic Pain (see text)

New Targets: The Claustrum

Targeting Teaser Winners

Recent Scientific References

• Hanganu IL, Staiger JF, Ben-Ari Y, Khazipov R (2007) Cholinergic modulation of spindle bursts in the neonatal rat visual cortex in vivo. J Neurosci 27:5694-5705. (Cat. #IT-01)
• Myhrer T, Enger S, Aas P (2007) Anticonvulsant effects of damage to structures involved in seizure induction in rats exposed to soman. Neurotoxicology. (Cat. #IT-01)
• Rahman W, Sikander S, Suzuki R, Hunt SP, Dickenson AH (2007) Superficial NK1 expressing spinal dorsal horn neurones modulate inhibitory neurotransmission mediated by spinal GABA(A) receptors. Neurosci Lett 419:278-283. (Cat. #IT-07)
• Gibbs RB, Johnson DA (2007) Cholinergic lesions produce task-selective effects on delayed matching to position and configural association learning related to response pattern and strategy. Neurobiol Learn Mem 88:19-32. (Cat. #IT-01)
• Wiley RG, Kline RHt, Vierck CJ, Jr. (2007) Anti-nociceptive effects of selectively destroying substance P receptor-expressing dorsal horn neurons using [Sar(9),Met(O(2))(11)]-substance P-saporin: Behavioral and anatomical analyses. Neuroscience 146:1333-1345. (Cat. #IT-07; Cat. #IT-11)
• Pascual J, Heinrichs SC (2007) Olfactory neophobia and seizure susceptibility phenotypes in an animal model of epilepsy are normalized by impairment of brain corticotropin releasing factor. Epilepsia 48:827-833. (Cat. #IT-13; Cat. #PR-01)
• Ferry B, Herbeaux K, Cosquer B, Traissard N, Galani R, Cassel JC (2007) Immunotoxic cholinergic lesions in the basal forebrain reverse the effects of entorhinal cortex lesions on conditioned odor aversion in the rat. Neurobiol Learn Mem 88:114-126. (Cat. #IT-01)

Recent Scientific References

• Gravitt K, Marson L (2007) Effect of the destruction of cells containing the serotonin reuptake transporter on urethrogenital reflexes. J Sex Med 4:322-330; discussion 330-321. (Cat. #IT-23)
• Schiltz JC, Sawchenko PE (2007) Specificity and generality of the involvement of catecholaminergic afferents in hypothalamic responses to immune insults. J Comp Neurol 502:455-467. (Cat. #IT-03; Cat. #PR-01; Cat. #IT-18)
• Hess PR, Barnes C, Woolard MD, Johnson MD, Cullen JM, Collins EJ, Frelinger JA (2007) Selective deletion of antigen-specific CD8+ T cells by MHC class I tetramers coupled to the type I ribosome-inactivating protein saporin. Blood 109:3300-3307. (Cat. #IT-27)
• Vera-Portocarrero LP, Zhang ET, King T, Ossipov MH, Vanderah TW, Lai J, Porreca F (2007) Spinal NK-1 receptor expressing neurons mediate opioid-induced hyperalgesia and antinociceptive tolerance via activation of descending pathways. Pain 129:35-45. (Cat. #IT-07; Cat. #PR-01)
• Bak SP, Walters JJ, Takeya M, Conejo-Garcia JR, Berwin BL (2007) Scavenger receptor-a-targeted leukocyte depletion inhibits peritoneal ovarian tumor progression. Cancer Res 67:4783-4789. (Cat. #IT-26)
• Traissard N, Herbeaux K, Cosquer B, Jeltsch H, Ferry B, Galani R, Pernon A, Majchrzak M, Cassel JC (2007) Combined damage to entorhinal cortex and cholinergic basal forebrain neurons, two early neurodegenerative features accompanying Alzheimer's disease: effects on locomotor activity and memory functions in rats. Neuropsychopharmacology 32:851-871. (Cat. #IT-01; Cat. #IT-02)

Targeting Talk

• Your targeted toxin data sheet gives the following instruction for disposal: “Care in disposal is mandatory; autoclaving or exposure to 1 M sodium hydroxide will inactivate the material. All labware that comes into contact with this material should be likewise treated.” I am wondering if I can deactivate saporin byusing 10% bleach or if everything has to be autoclaved?
• I was wondering if there is any indication that intrastriatal administration of 192-IgG-SAP(Cat. #IT-01) will lesion the cholinergic neurons of the striatum. My sense from reviewing the literature is that these cholinergic neurons are not susceptible to the toxin, but I thought I'd ask to see if you had any information / experience regarding this point.
• I have a few questions about the Alexa488-labeled affinity-purified NGFr antibody (Cat.#FL-03). Is it specific to extracellular p75? Can you use it on live cells? Does it work onfixed cells? Does it cause activation of the p75 receptor (i.e., result in apoptosis or changes in axonoutgrowth in neuronal cells)?
• I purchased your secondary conjugate, Mab-ZAP (Cat. #IT-04). I am preparing to do a cytotoxicity assay and I'm wondering if my primary antibody should be sterilized prior to combining with Mab-ZAP?

Upcoming Events

• Society for Neuroscience, San Diego CA — November 3-7, 2007
• American Society for Cell Biology , Washington DC — December 1-5, 2007

Flow Cytometry Services (www.CytometryRes.com)

Targeting Tools: New Products

• Anti-CD22 (UV22-2) Mouse Monoclonal (Cat. #AB-36)
• Mouse IgG-FITC (Cat. #FL-07)
• Anti-CD22-SAP (Cat. #IT-37)

Gangsta Gallery

Targeting Teaser (Jumble)