Secondary Antibody Conjugate References

2008

Siva AC, Wild MA, Kirkland RE, Nolan MJ, Lin B, Maruyama T, Yantiri-Wernimont F, Frederickson S, Bowdish KS, Xin H (2008) Targeting CUB domain-containing protein 1 with a monoclonal antibody inhibits metastasis in a prostate cancer model. Cancer Res 68:3759-3766. (Targeting Trends summary)

Zhao XY, Liu HL, Liu B, Willuda J, Siemeister G, Mahmoudi M, Dinter H (2008) Tomoregulin internalization confers selective cytotoxicity of immunotoxins on prostate cancer cells. Transl Oncol 1:102-109. (Targeting Trends summary)

2007

Bak SP, Walters JJ, Takeya M, Conejo-Garcia JR, Berwin BL (2007) Scavenger receptor-a-targeted leukocyte depletion inhibits peritoneal ovarian tumor progression. Cancer Res 67:4783-4789. (Targeting Trends summary)

2006

Adam PJ, Terrett JA, Steers G, Stockwin L, Loader JA, Fletcher GC, Lu LS, Leach BI, Mason S, Stamps AC, Boyd RS, Pezzella F, Gatter KC, Harris AL (2006) CD70 (TNFSF7) is expressed at high prevalence in renal cell carcinomas and is rapidly internalised on antibody binding. Br J Cancer 95(3):298-306. (Targeting Trends summary)

Foehr ED, Lorente G, Kuo J, Ram R, Nikolich K, Urfer R (2006) Targeting of the receptor protein tyrosine phosphatase beta with a monoclonal antibody delays tumor growth in a glioblastoma model. Cancer Res 66(4):2271-2278. (Targeting Trends summary)

Fransson J, Borrebaeck CA (2006) The nuclear DNA repair protein Ku70/80 is a tumor-associated antigen displaying rapid receptor mediated endocytosis. Int J Cancer 119(10):2492-2496. (Targeting Trends summary)

Nguyen DH, Ball ED, Varki A (2006) Myeloid precursors and acute myeloid leukemia cells express multiple CD33-related Siglecs. Exp Hematol 34(6):728-735. (Targeting Trends summary)

2000

Kohls MD, Lappi DA (2000) Mab-ZAP: A tool for evaluating antibody efficacy for use in an immunotoxin. BioTechniques 28(1):162-165.

Protocol:
The assay for eliminating targeted cells, described in Kohls et al. (2000), uses the material listed below.

the secondary conjugate	your primary antibody
your cell line	medium
96 well plate (VWR #29442-054)	PMS (Sigma #P-9625, 5 g)
MTS (Promega #G1112, 240 mg)

1992

Weltman JK, Melucci CL, Chen J, Davidson AE (1992) Internalization of monoclonal antibodies selected for immunotoxin activity against small-cell lung cancer. Hybridoma 1:547-559.

1989

Barbieri L, Dinota A, Lappi DA, Soria M, Tazzari PL, Stirpe F (1989) Selective killing of CD4+ and CD8+ cells with immunotoxins containing saporin. Scand J Immunol 30:369-372.

1987

Weltman JK, Pedroso P, Johnson S, Davignon D, Fast LD, Leone LA (1987) Rapid screening with indirect immunotoxin for monoclonal antibodies against human small cell lung cancer. Cancer Res 47(21):5552-5556.