FabFc-ZAP Hug-M [IT-78, KIT-78]

a tool to “piggyback” onto YOUR antibody via goat anti-human IgM monovalent antibody; targeting cells that recognize YOUR human IgM antibody, eliminated via saporin

SKU: IT-78 Category: Quantity: Individual 25 ug, Individual 100 ug, Individual 250 ug, Individual 1 mg, Kit w/controls 25 ug, Kit w/controls 100 ug, Kit w/controls 250 ug, Kit (1 ab) w/controls & developing reagents, tests 1 ab, Kit (4 ab) w/controls & developing reagents, tests 4 abs, Kit (10 ab) w/controls & developing reagents, tests 10 abs | Antibody Type: affinity-purified | Host: goat | Reactivity: human | Conjugate: saporin | Usage: eliminates cells, screen antibodies |

FabFc-ZAP Hug-M uses your primary human monoclonal IgM antibody to target and eliminate cells that recognize your primary antibody. The secondary antibody used in FabFc-ZAP Hug-M will react only with the Fc portion of the IgM heavy chain. When the in vitro results confirm the desired specificity, it is recommended that you order a custom conjugation of your antibody to saporin.

FabFc-ZAP Hug-M is a chemical conjugate of goat anti-human IgM antibody and the ribosome-inactivating protein, saporin. It uses your human primary IgM antibody to target and eliminate cells. This secondary conjugate is used to evaluate the potential of a primary antibody to internalize.

FabFc-ZAP Hug-M is available individually (Cat. #IT-78) or as a kit (Cat. #KIT-78) which includes FabFc-ZAP Hug-MSaporin (Cat. #PR-01)Fab IgG-SAP (Cat. #IT-67) and reagents for developing a cytotoxicity assay.

Other ZAP Conjugates:

Need another ZAP Conjugate for your target? Check here for other species & targets including in vivo and in vitro options.

keywords: antibody, efficacy, immunotoxin, screening, internalization, saporin, Fab-ZAP, FabFc-ZAP, monovalent Fc region, secondary conjugate, primary antibody, anti-human IgM

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Mab-ZAP: A tool for evaluating antibody efficacy for use in an immunotoxin.

Kohls MD, Lappi DA (2000) Mab-ZAP: A tool for evaluating antibody efficacy for use in an immunotoxin. BioTechniques 28(1):162-165. doi: 10.2144/00281pf01

Summary: Immunotoxins are useful tools for elimination of specific cell populations in vitro and in vivo for research and therapeutic applications. One of the factors limiting the use of immunotoxins is the selection of an appropriate antibody. Advanced Targeting Systems has created a reagent that allows researchers to select antibodies with the desired characteristics before an immuntoxin is made, purified, and assayed. Using a goat anti-murine IgG coupled to the ribosome-inactivating protein saporin, researchers can screen hundreds of antibodies in a time and cost-effective manner.

Related Products: Mab-ZAP (Cat. #IT-04), Rab-ZAP (Cat. #IT-05), Hum-ZAP (Cat. #IT-22), Rat-ZAP (Cat. #IT-26), Anti-M-ZAP (Cat. #IT-30), Goat-ZAP (Cat. #IT-36)


Streptavidin-saporin: Converting biotinylated materials into targeted toxins

Ancheta LR, Shramm PA, Bouajram R, Higgins D, Lappi DA (2023) Streptavidin-saporin: Converting biotinylated materials into targeted toxins. Toxins 15(3):181. doi: 10.3390/toxins15030181

Summary: This manuscript describes the myriad of ways Streptavidin-ZAP is used and how this technology supports the scientific process of ‘Molecular Surgery’ and progress in research and drug development. Insights from publications and research performed using Streptavidin-ZAP and its impact on academia and industry for research and drug development are presented.

Read the full article.

Related Products: Streptavidin-ZAP (Cat. #IT-27)


Saporin as a commercial reagent: its uses and unexpected impacts in the biological sciences-tools from the plant kingdom

Ancheta LR, Shramm PA, Bouajram R, Higgins D, Lappi DA (2022) Saporin as a commercial reagent: its uses and unexpected impacts in the biological sciences-tools from the plant kingdom. Toxins (Basel) 14(3):184. doi: 10.3390/toxins14030184

Read complete article.


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Fab IgG-SAP (Cat. #IT-67)

FabFc-ZAP human (Cat. #IT-65)

FabFc-ZAP Hug-M (Cat. #IT-78)

FabFc-ZAP mouse (Cat. #IT-89)

FabFc-ZAP rat (Cat. #IT-88)

FabFc-pHast HugM (Cat. #PH-04)

Saporin (Cat. #PR-01)

Hug-M-ZAP (Cat. #IT-43)

Targeting Tools: FabFc-ZAP – A new secondary conjugate

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FabFc-ZAP cross-reaction with another species

Targeting B Cells

ZAP Antibody Internalization Kits

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Dosage of Fab-ZAP for antibody concentration

Recommended ratio between Fab-ZAP dosage and antibody concentration

Which secondary ZAP conjugate should I use?

Fab-ZAP number of replications

ZAP Antibody Internalization Kit Introduction

ZAP Antibody Internalization Kit Tutorial

ZAP Antibody Internalization Kit literature

Cytotoxicity Assay Protocol for ZAP Antibody Internalization Kits

Online Calculator: Preparing ZAP Antibody Kit Samples

Concentration Calculations: Convert molarity to mg/ml and mg/ml to molarity (PDF worksheet)

Preparing and Interpreting Cytotoxicity Data in vitro


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