Alpaca-ZAP [IT-87, KIT-87]

a tool to “piggyback” onto YOUR antibody via goat anti-alpaca IgG (VHH Domain); targeting cells that recognize YOUR primary antibody, eliminated via saporin

SKU: IT-87 Category: Quantity: Individual 25 ug, Individual 100 ug, Individual 250 ug, Individual 1 mg, Kit w/controls 25 ug, Kit w/controls 100 ug, Kit w/controls 250 ug, Kit (1 ab) w/controls & developing reagents, tests 1 ab, Kit (4 ab) w/controls & developing reagents, tests 4 abs, Kit (10 ab) w/controls & developing reagents, tests 10 abs | Host: goat | Reactivity: alpaca, llama | Conjugate: saporin | Usage: eliminates cells, screen antibodies |

Alpaca-ZAP recognizes YOUR primary antibody. It is a chemical conjugate of goat anti-alpaca IgG (VHH domain) and the ribosome-inactivating protein, saporin. The antibodies used to make Alpaca-ZAP mouse are affinity-purified against the heavy chain of alpaca IgG (VHH domain). The antibody used in this product reacts with the VHH domain of heavy chain (HC) alpaca IgG, subclasses 2 and 3, and with the VHH domain of llama IgG, subclasses 2 and 3.  It also reacts with whole molecule alpaca and llama IgG1.

Alpaca-ZAP is a chemical conjugate of goat anti-alpaca IgG (VHH domain) and the ribosome-inactivating protein, saporin. It uses your mouse primary antibody to target and eliminate cells. This secondary conjugate is used to evaluate the potential of a primary antibody to internalize.

Alpaca-ZAP is available individually (Cat. #IT-87) or as a kit (Cat. #KIT-87) which includes Alpaca-ZAPSaporin (Cat. #PR-01)Goat IgG-SAP (Cat. #IT-19) and reagents for developing a cytotoxicity assay.

Other ZAP Conjugates:

Need another ZAP Conjugate for your target? Check here for other species & targets including in vivo and in vitro options.

keywords: Alpaca Antibody, Alpaca Primary, Anti-Alpaca IgG, Alpaca IgG, Llama, Llama IgG1, VHH domain, heavy chain, Screening, ADC, Antibody Drug Conjugate, in vitro, Secondary

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View Data Sheet

Evaluate Potential Targeting Molecules.

Kohls M (2006) Evaluate Potential Targeting Molecules. Nature Methods

Summary: Targeted toxins -- targeting agents conjugated to saporin -- are widely used to eliminate specific cell populations both in vitro and in vivo. For these molecules to be effective, it is vital that the targeting component of the conjugate specifically binds the cells of interest. A secondary conjugate, Streptavidin-ZAP, has been created by attaching the toxin saporin to streptavidin. The user can combine primary biotinylated material with Streptavidin-ZAP to quickly and economically screen potential targeting molecules for internalization and specificity. Once the appropriate targeting molecule is identified, a direct conjugation with saporin can be performed.

Related Products: Streptavidin-ZAP (Cat. #IT-27)

Read the article.

Saporin as a commercial reagent: its uses and unexpected impacts in the biological sciences-tools from the plant kingdom

Ancheta LR, Shramm PA, Bouajram R, Higgins D, Lappi DA (2022) Saporin as a commercial reagent: its uses and unexpected impacts in the biological sciences-tools from the plant kingdom. Toxins (Basel) 14(3):184. doi: 10.3390/toxins14030184

Read complete article.

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Saporin (Cat. #PR-01)

Goat IgG-SAP (Cat. #IT-19)

Alpaca-pHast (Cat. #PH-05)

Choosing the Correct Secondary Conjugate

ZAP Antibody Internalization Kit Introduction

ZAP Antibody Internalization Kit Tutorial

ZAP Antibody Internalization Kit literature

Cytotoxicity Assay Protocol for ZAP Antibody Internalization Kits

Online Calculator: Preparing ZAP Antibody Kit Samples

Concentration Calculations: Convert molarity to mg/ml and mg/ml to molarity (PDF worksheet)

Preparing and Interpreting Cytotoxicity Data in vitro


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